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Mouse Anti-USP44 (aa 420-712) Recombinant Antibody (1A9) (CBMAB-U0017-CQ)

This product is a mouse antibody that recognizes USP44 (aa 420-712). The antibody 1A9 can be used for immunoassay techniques such as: IHC, WB.
See all USP44 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1A9
Antibody Isotype
IgG2a
Application
IHC, WB

Basic Information

Immunogen
Recombinant protein fragment between amino acids 420-712 of human USP44 produced in E.coli
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/mL
Purity
>95% as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 420-712

Target

Full Name
Ubiquitin specific peptidase 44
Introduction
The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset.
Entrez Gene ID
84101
UniProt ID
Q9H0E7
Alternative Names
Ubiquitin Specific Peptidase 44; Ubiquitin Specific Protease 44
Function
Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.
Biological Process
Biological Process cell cycle Source:UniProtKB-KW
Biological Process cell division Source:UniProtKB-KW
Biological Process chromosome segregation Source:Ensembl
Biological Process negative regulation of ubiquitin protein ligase activity Source:UniProtKB1 Publication
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:UniProtKB
Biological Process protein deubiquitination Source:UniProtKB2 Publications
Biological Process regulation of mitotic cell cycle spindle assembly checkpoint Source:UniProtKB
Cellular Location
Nucleus
Peaks in interphase, with relatively low levels maintained throughout mitosis.
PTM
Dephosphorylated by CTDP1.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination and is degraded by the proteasome.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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