Mouse Anti-USP44 (aa 420-712) Recombinant Antibody (1C2) (CBMAB-U0022-CQ)

Name: Mouse Anti-USP44 (aa 420-712) Recombinant Antibody (1C2)
SKU: CBMAB-U0022-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1C2

Application

Immunogen

Recombinant protein fragment between amino acids 420-712 of human USP44 produced in E.coli

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/mL

Purity

>95% as determined by SDS-PAGE analysis

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 420-712

More Infomation

Target

Full Name

Ubiquitin specific peptidase 44

Introduction

The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset.

Entrez Gene ID

84101

UniProt ID

Q9H0E7

Alternative Names

Ubiquitin Specific Peptidase 44; Ubiquitin Specific Protease 44

Function

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.

Biological Process

Biological Process cell cycle Source:UniProtKB-KW
Biological Process cell division Source:UniProtKB-KW
Biological Process chromosome segregation Source:Ensembl
Biological Process negative regulation of ubiquitin protein ligase activity Source:UniProtKB1 Publication
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic process Source:UniProtKB
Biological Process protein deubiquitination Source:UniProtKB2 Publications
Biological Process regulation of mitotic cell cycle spindle assembly checkpoint Source:UniProtKB

Cellular Location

Nucleus
Peaks in interphase, with relatively low levels maintained throughout mitosis.

PTM

Dephosphorylated by CTDP1.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination and is degraded by the proteasome.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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