Mouse Anti-VRK1 Recombinant Antibody (1F6) (CBMAB-V0061-YC)

Name: Mouse Anti-VRK1 Recombinant Antibody (1F6)
SKU: CBMAB-V0061-YC
Rating: 5 (1 reviews)

See all VRK1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1F6

Application

WB, IHC, ICC, IF, IHC-P

Immunogen

Recombinant fusion protein GST-VRK1

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Vaccinia Related Kinase 1

Introduction

VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. VRK1 is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. VRK1 localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. VRK1 may regulate cell proliferation.

Entrez Gene ID

Human	7443
Mouse	22367

UniProt ID

Human	Q99986
Mouse	Q80X41

Alternative Names

Vaccinia Related Kinase 1; EC 2.7.11.1; Serine/Threonine-Protein Kinase VRK1; Vaccinia Virus B1R-Related Kinase 1; Vaccinia-Related Kinase 1; PCH1A; PCH1

Function

Serine/threonine kinase involved in cell cycle, nuclear condensation and transcription regulation (PubMed:14645249, PubMed:18617507, PubMed:19103756).
Involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation (PubMed:19103756).
Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2 (PubMed:10951572).
Phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed:33076429).
Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed:16495336).
Phosphorylates ATF2 which activates its transcriptional activity (PubMed:15105425).

Biological Process

Biological Process cell division Source:UniProtKB-KW
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process Golgi disassembly Source:UniProtKB1 Publication
Biological Process mitotic nuclear membrane disassembly Source:Reactome
Biological Process peptidyl-serine phosphorylation Source:GO_Central1 Publication
Biological Process positive regulation of protein localization to chromatin Source:UniProtKB1 Publication
Biological Process protein autophosphorylation Source:UniProtKB2 Publications
Biological Process protein phosphorylation Source:ProtInc1 Publication
Biological Process signal transduction Source:GO_Central1 Publication

Cellular Location

Nucleus
Cytoplasm
Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.

Involvement in disease

Pontocerebellar hypoplasia 1A (PCH1A):
A disorder characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and spinal cord anterior horn cells degeneration resembling infantile spinal muscular atrophy. Additional features include muscle hypotonia, congenital contractures and respiratory insufficiency that is evident at birth.

PTM

Autophosphorylated at various serine and threonine residues (PubMed:11883897, PubMed:14645249, PubMed:19103756, PubMed:21543316).
Autophosphorylation does not impair its ability to phosphorylate p53/TP53 (PubMed:11883897).
Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis (PubMed:19103756).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-VRK1 Recombinant Antibody (1F6)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

VRK1 Matched Antibody Pair (1303) (CAT#: APMAB-1303LY)

Related Products

Mouse Anti-VRK1 Recombinant Antibody (2C9) (CAT#: CBMAB-V0056-YC)

Mouse Anti-VRK1 Recombinant Antibody (5D1) (CAT#: CBMAB-V0059-YC)

VRK1 Matched Antibody Pair (1303) (CAT#: APMAB-1303LY)

Mouse Anti-VRK1 Recombinant Antibody (2E10) (CAT#: CBMAB-V0062-YC)

Mouse Anti-VRK1 Recombinant Antibody (4F9) (CAT#: CBMAB-V0057-YC)

Mouse Anti-VRK1 Recombinant Antibody (CBXS-0943) (CAT#: CBMAB-S3857-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry