Mouse Anti-WEE1 Recombinant Antibody (CBWJW-019) (CBMAB-W0176-WJ)

Name: Mouse Anti-WEE1 Recombinant Antibody (CBWJW-019)
SKU: CBMAB-W0176-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBWJW-019

Application

WB, IP

Specificity

Human

Antibody Isotype

IgM

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

0.316 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

WEE1 G2 Checkpoint Kinase

Introduction

WEE1 is a protein-coding gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. Diseases associated with WEE1 include Beckwith-Wiedemann Syndrome. The related pathways are Response to elevated platelet cytosolic Ca2+ and ERK Signaling. An important paralog of this gene is WEE2.

Entrez Gene ID

7465

UniProt ID

P30291

Alternative Names

WEE1A; WEE1hu

Function

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 ^oCcurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Biological Process

Cell divisionIEA:UniProtKB-KW
Establishment of cell polarityIEA:Ensembl
G2/M transition of mitotic cell cycleTAS:Reactome
Microtubule cytoskeleton organizationIEA:Ensembl
Negative regulation of G1/S transition of mitotic cell cycleTAS:Reactome
Neuron projection morphogenesisIEA:Ensembl

Cellular Location

Nucleus

PTM

Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.
Dephosphorylated at Thr-239 by CTDP1 (PubMed:22692537).
Dephosphorylated at Ser-53 and Ser-123 by the serine/threonine-protein phosphatase 2A preventing its ubiquitin-mediated degradation (PubMed:33108758).
Ubiquitinated and degraded at the onset of G2/M phase.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-WEE1 Recombinant Antibody (CBWJW-020) (CAT#: CBMAB-W0177-WJ)

Mouse Anti-WEE1 Recombinant Antibody (CBWJW-022) (CAT#: CBMAB-W0179-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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