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Rabbit Anti-WEE1 Recombinant Antibody (CBWJW-020) (CBMAB-W0177-WJ)

This product is a rabbit antibody that recognizes WEE1. The antibody CBWJW-020 can be used for immunoassay techniques such as: FC.
See all WEE1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
CBWJW-020
Application
FC

Basic Information

Specificity
Human, Monkey
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
WEE1 G2 Checkpoint Kinase
Introduction
WEE1 is a protein-coding gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. Diseases associated with WEE1 include Beckwith-Wiedemann Syndrome. The related pathways are Response to elevated platelet cytosolic Ca2+ and ERK Signaling. An important paralog of this gene is WEE2.
Entrez Gene ID
Human7465
Monkey703760
UniProt ID
HumanP30291
MonkeyF7DQS9
Alternative Names
WEE1A; WEE1hu
Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 oCcurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Biological Process
Cell divisionIEA:UniProtKB-KW
Establishment of cell polarityIEA:Ensembl
G2/M transition of mitotic cell cycleTAS:Reactome
Microtubule cytoskeleton organizationIEA:Ensembl
Negative regulation of G1/S transition of mitotic cell cycleTAS:Reactome
Neuron projection morphogenesisIEA:Ensembl
Cellular Location
Nucleus
PTM
Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.
Dephosphorylated at Thr-239 by CTDP1 (PubMed:22692537).
Dephosphorylated at Ser-53 and Ser-123 by the serine/threonine-protein phosphatase 2A preventing its ubiquitin-mediated degradation (PubMed:33108758).
Ubiquitinated and degraded at the onset of G2/M phase.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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