Rabbit Anti-YWHAG Recombinant Antibody (
13C9) (V2LY-0725-LY1389)

Name: Rabbit Anti-YWHAG Recombinant Antibody (13C9)
SKU: V2LY-0725-LY1389
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-YWHAG Recombinant Antibody (13C9) (V2LY-0725-LY1389) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, K562 whole cell lysate
All lanes: YWHAG antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa

Rabbit Anti-YWHAG Recombinant Antibody (13C9) (V2LY-0725-LY1389) in IHC

IHC image of V2LY-0725-LY1389 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

13C9

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human YWHAG.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide

Entrez Gene ID

7532

UniProt ID

P61981

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Biological Process

Biological Process cellular response to insulin stimulus Source:Ensembl
Biological Process negative regulation of protein kinase activity Source:UniProtKB1 Publication
Biological Process protein localization Source:GO_Central1 Publication
Biological Process protein targeting Source:Ensembl
Biological Process regulation of neuron differentiation Source:UniProtKB1 Publication
Biological Process regulation of signal transduction Source:UniProtKB2 Publications
Biological Process regulation of synaptic plasticity Source:UniProtKB1 Publication
Biological Process signal transduction Source:GO_Central1 Publication

Cellular Location

Cytoplasm

Involvement in disease

Developmental and epileptic encephalopathy 56 (DEE56):
A form of epileptic encephalopathy, a heterogeneous group of severe early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE56 is an autosomal dominant condition.

PTM

Phosphorylated by various PKC isozymes.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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