Canine Recombinant IL25 protein, hFc Tag (V2LY-0526-LY1282)

Name: Canine Recombinant IL25 protein, hFc Tag
SKU: V2LY-0526-LY1282
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Canine

Tag

hFc Tag

Protein Construction

This product is Canine Recombinant IL25 protein, hFc Tag consist of Amino Acid: 17-169 and predicts a molecular mass of 46.2 kDa.

Molecule Mass

46.2 kDa

Sequence

Amino Acid: 17-169

Species

Canine

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Interleukin 25

Function

Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Biological Process

Eosinophil differentiationIEA:Ensembl
Inflammatory response to antigenic stimulusIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Response to fungusIEA:Ensembl
Response to nematodeIEA:Ensembl

Cellular Location

Secreted

Chen, J., Wang, Y., Shen, L., Xiu, Y., & Wang, B. (2023). Could IL-25 be a potential therapeutic target for intestinal inflammatory diseases?. Cytokine & Growth Factor Reviews, 69, 43-50.

Jou, E., Rodriguez-Rodriguez, N., Ferreira, A. C. F., Jolin, H. E., Clark, P. A., Sawmynaden, K., ... & McKenzie, A. N. (2022). An innate IL-25–ILC2–MDSC axis creates a cancer-permissive microenvironment for Apc mutation–driven intestinal tumorigenesis. Science immunology, 7(72), eabn0175.

Zhang, K., Feng, Y., Liang, Y., Wu, W., Chang, C., Chen, D., ... & Zhen, G. (2021). Epithelial miR-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2–high asthma. JCI insight, 6(11).

Borowczyk, J., Shutova, M., Brembilla, N. C., & Boehncke, W. H. (2021). IL-25 (IL-17E) in epithelial immunology and pathophysiology. Journal of Allergy and Clinical Immunology, 148(1), 40-52.

Deng, C., Peng, N., Tang, Y., Yu, N., Wang, C., Cai, X., ... & Lu, L. (2021). Roles of IL-25 in type 2 inflammation and autoimmune pathogenesis. Frontiers in Immunology, 12, 691559.

Ualiyeva, S., Lemire, E., Aviles, E. C., Wong, C., Boyd, A. A., Lai, J., ... & Bankova, L. G. (2021). Tuft cell–produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation. Science immunology, 6(66), eabj0474.

Leyva-Castillo, J. M., Galand, C., Mashiko, S., Bissonnette, R., McGurk, A., Ziegler, S. F., ... & Geha, R. S. (2020). ILC2 activation by keratinocyte-derived IL-25 drives IL-13 production at sites of allergic skin inflammation. Journal of Allergy and Clinical Immunology, 145(6), 1606-1614.

Hiraishi, Y., Yamaguchi, S., Yoshizaki, T., Nambu, A., Shimura, E., Takamori, A., ... & Nakae, S. (2018). IL-33, IL-25 and TSLP contribute to development of fungal-associated protease-induced innate-type airway inflammation. Scientific reports, 8(1), 18052.

Bornstein, C., Nevo, S., Giladi, A., Kadouri, N., Pouzolles, M., Gerbe, F., ... & Amit, I. (2018). Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells. Nature, 559(7715), 622-626.

Bankova, L. G., Dwyer, D. F., Yoshimoto, E., Ualiyeva, S., McGinty, J. W., Raff, H., ... & Barrett, N. A. (2018). The cysteinyl leukotriene 3 receptor regulates expansion of IL-25–producing airway brush cells leading to type 2 inflammation. Science immunology, 3(28), eaat9453.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IL25 protein, hFc Tag (CAT#: V2LY-0526-LY4865)

Mouse Recombinant IL25 protein, His Tag (CAT#: V2LY-0526-LY8522)

Rat Recombinant IL25 protein, hFc Tag (CAT#: V2LY-0526-LY9469)

Human IL25 ELISA Kit (CAT#: V2LY-0626-LY264)

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Human IL25 ELISA Kit (CAT#: V2LY-0626-LY264)

Rat Anti-IL25 Recombinant Antibody (CBYY-I1075) (CAT#: CBMAB-I2243-YY)

Human Recombinant IL25 protein, hFc Tag (CAT#: V2LY-0526-LY4865)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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