Human IL25 ELISA Kit (V2LY-0626-LY264)

Name: Human IL25 ELISA Kit
SKU: V2LY-0626-LY264
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.011 ng/mL

Detection Range

0.02-6 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

20.3 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Interleukin 25

Function

Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Biological Process

Eosinophil differentiationIEA:Ensembl
Inflammatory response to antigenic stimulusIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Response to fungusIEA:Ensembl
Response to nematodeIEA:Ensembl

Cellular Location

Secreted

Chen, J., Wang, Y., Shen, L., Xiu, Y., & Wang, B. (2023). Could IL-25 be a potential therapeutic target for intestinal inflammatory diseases?. Cytokine & Growth Factor Reviews, 69, 43-50.

Jou, E., Rodriguez-Rodriguez, N., Ferreira, A. C. F., Jolin, H. E., Clark, P. A., Sawmynaden, K., ... & McKenzie, A. N. (2022). An innate IL-25–ILC2–MDSC axis creates a cancer-permissive microenvironment for Apc mutation–driven intestinal tumorigenesis. Science immunology, 7(72), eabn0175.

Zhang, K., Feng, Y., Liang, Y., Wu, W., Chang, C., Chen, D., ... & Zhen, G. (2021). Epithelial miR-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2–high asthma. JCI insight, 6(11).

Borowczyk, J., Shutova, M., Brembilla, N. C., & Boehncke, W. H. (2021). IL-25 (IL-17E) in epithelial immunology and pathophysiology. Journal of Allergy and Clinical Immunology, 148(1), 40-52.

Deng, C., Peng, N., Tang, Y., Yu, N., Wang, C., Cai, X., ... & Lu, L. (2021). Roles of IL-25 in type 2 inflammation and autoimmune pathogenesis. Frontiers in Immunology, 12, 691559.

Ualiyeva, S., Lemire, E., Aviles, E. C., Wong, C., Boyd, A. A., Lai, J., ... & Bankova, L. G. (2021). Tuft cell–produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation. Science immunology, 6(66), eabj0474.

Leyva-Castillo, J. M., Galand, C., Mashiko, S., Bissonnette, R., McGurk, A., Ziegler, S. F., ... & Geha, R. S. (2020). ILC2 activation by keratinocyte-derived IL-25 drives IL-13 production at sites of allergic skin inflammation. Journal of Allergy and Clinical Immunology, 145(6), 1606-1614.

Hiraishi, Y., Yamaguchi, S., Yoshizaki, T., Nambu, A., Shimura, E., Takamori, A., ... & Nakae, S. (2018). IL-33, IL-25 and TSLP contribute to development of fungal-associated protease-induced innate-type airway inflammation. Scientific reports, 8(1), 18052.

Bornstein, C., Nevo, S., Giladi, A., Kadouri, N., Pouzolles, M., Gerbe, F., ... & Amit, I. (2018). Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells. Nature, 559(7715), 622-626.

Bankova, L. G., Dwyer, D. F., Yoshimoto, E., Ualiyeva, S., McGinty, J. W., Raff, H., ... & Barrett, N. A. (2018). The cysteinyl leukotriene 3 receptor regulates expansion of IL-25–producing airway brush cells leading to type 2 inflammation. Science immunology, 3(28), eaat9453.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant IL25 protein, hFc Tag (CAT#: V2LY-0526-LY1282)

Human Recombinant IL25 protein, hFc Tag (CAT#: V2LY-0526-LY4865)

Mouse Recombinant IL25 protein, His Tag (CAT#: V2LY-0526-LY8522)

Rat Recombinant IL25 protein, hFc Tag (CAT#: V2LY-0526-LY9469)

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Canine Recombinant IL25 protein, hFc Tag (CAT#: V2LY-0526-LY1282)

Rat Anti-IL25 Recombinant Antibody (CBYY-I0192) (CAT#: CBMAB-I0521-YY)

Rat Anti-IL25 Recombinant Antibody (207702) (CAT#: CBMAB-I0520-YY)

Mouse Anti-IL25 Recombinant Antibody (8B1) (CAT#: CBMAB-I1748-YY)

Rat Anti-IL25 Recombinant Antibody (CBYY-I0448) (CAT#: CBMAB-I1048-YY)

Mouse Anti-IL25 Recombinant Antibody (CBYY-I1126) (CAT#: CBMAB-I2294-YY)

Mouse Anti-IL25 Recombinant Antibody (6D502) (CAT#: CBMAB-I1563-YY)

Mouse Recombinant IL25 protein, His Tag (CAT#: V2LY-0526-LY8522)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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