Cattle ITGB5 ELISA Kit (V2LY-0626-LY2556)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0093 ng/mL
Detection Range
0.02-3.8 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Cattle
Assay Type
Sandwich
Reactivity
Cattle
Assay Time
1.5 h
Molecule Mass
88.1 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Integrin Subunit Beta 5
Function
Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.
(Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C.
Biological Process
Cell adhesion mediated by integrinManual Assertion Based On ExperimentIBA:GO_Central
Cell migrationManual Assertion Based On ExperimentIMP:BHF-UCL
Cell-matrix adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Endodermal cell differentiationManual Assertion Based On ExperimentIEP:UniProtKB
Epithelial cell-cell adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Stress fiber assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Membrane
Topology
Extracellular: 24-719
Helical: 720-742
Cytoplasmic: 743-799

Gu, L., Jin, X., Liang, H., Yang, C., & Zhang, Y. (2023). Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex. Pharmacological Research, 192, 106789.

Liu, D., Liu, S., Fang, Y., Liu, L., & Hu, K. (2022). Comprehensive analysis of the expression and prognosis for ITGBs: identification of ITGB5 as a biomarker of poor prognosis and correlated with immune infiltrates in gastric cancer. Frontiers in Cell and Developmental Biology, 9, 816230.

Wen, X., Chen, S., Chen, X., Qiu, H., Wang, W., Zhang, N., ... & Zhang, L. (2022). ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway. Frontiers in Oncology, 12, 887068.

Yang, Y., Feng, Q., Hu, K., & Cheng, F. (2021). Using CRISPRa and CRISPRi technologies to study the biological functions of ITGB5, TIMP1, and TMEM176B in prostate cancer cells. Frontiers in Molecular Biosciences, 8, 676021.

Wang, W., Liu, Y., Tang, H., Yu, Y., & Zhang, Q. (2019). ITGB5 plays a key role in Escherichia coli F4ac-induced diarrhea in piglets. Frontiers in Immunology, 10, 2834.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

ITGB5 Matched Antibody Pair (652) (CAT#: APMAB-652LY)

Antibody Pairs

ITGB5 Matched Antibody Pair (652) (CAT#: APMAB-652LY)

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ITGB5 Matched Antibody Pair (652) (CAT#: APMAB-652LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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