Anti-CD274 Antibody (CBMAB-C1251-FY) Immunohistochemistry Kit (V2LY-0826-LY202)

Name: Anti-CD274 Antibody (CBMAB-C1251-FY) Immunohistochemistry Kit
SKU: V2LY-0826-LY202
Rating: 5 (1 reviews)

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Datasheet

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Summary

Product Overview

This comprehensive, all-in-one kit is designed for the precise immunohistochemical (IHC) detection of target protein in tissue samples. Unlike standard detection kits, it includes a highly specific mouse primary antibody bundled directly with our robust Polymer HRP detection system. The kit provides every essential reagent needed for the entire IHC workflow-from the initial antigen retrieval buffer to the final mounting media. By offering a pre-matched primary antibody with optimized buffers and secondary detection reagents, this kit eliminates the need for tedious protocol optimization and reagent sourcing, ensuring consistent, high-quality staining results.

Primary Antibody Cat.

CBMAB-C1251-FY

Primary Antibody

Mouse Anti-CD163 Recombinant Antibody (RM3/1)

Secondary Antibody Host Species

Goat

Secondary Antibody Species Specificity

Mouse

Application

IHC-P

Sample Type

FFPE tissue

Test Size

50 Tests

Storage

Stored at 2-8°C. The kit is stable for 6 months from the date of receipt.

Key Features

Target-Specific Primary Antibody

Includes a highly validated mouse primary antibody, ensuring reliable and specific target localization.

Complete End-to-End Solution

Contains all 11 essential components required for a full IHC workflow, including Tris-EDTA Antigen Retrieval Buffer, Blocking Buffer, Polymer HRP-Goat anti-Mouse Secondary Antibody, DAB Chromogens, Counterstain, and Mounting Media.

Streamlined Workflow

Pre-optimized reagents work seamlessly with the included primary antibody, saving researchers significant time on antibody titration and protocol adjustments.

Sample Compatibility

Highly recommended and optimized for Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.

Kit Capacity

Provides sufficient reagents (50T) to perform 50 tests, depending on tissue section size.

More Infomation

Sub Cat	Reagents	Size
LV01	Antigen Retrieval Buffer (50× Tris-EDTA)	100 ml
LV02	Washing Buffer (30×)	120 ml
LV03	Blocking Buffer	5 ml
LV04	Quenching buffer (RTU)	5 ml
LV05	Primary Antibody Diluent (RTU)	100 ml
LV06M	HRP-Goat anti-Mouse Secondary Antibody	5 ml
LV07	Chromogen Component A	0.2 ml
LV08	Chromogen Component B	4 ml
LV09	Signal Enhancer	5 ml
LV10	Counter Staining Reagent	5 ml
LV11	Mounting Media	5 ml

Verocq, C., Decaestecker, C., Rocq, L., De Clercq, S., Verrellen, A., Mekinda, Z., ... & D'haene, N. (2020). The daily practice reality of PD‑L1 (CD274) evaluation in non‑small cell lung cancer: A retrospective study. Oncology letters, 19(5), 3400-3410.

Riemann, D., Schütte, W., Turzer, S., Seliger, B., & Möller, M. (2020). High PD-L1/CD274 Expression of Monocytes and Blood Dendritic Cells Is a Risk Factor in Lung Cancer Patients Undergoing Treatment with PD1 Inhibitor Therapy. Cancers, 12(10), 2966.

Kim, J. Y., Lee, K. H., Kang, J., Borcoman, E., Saada-Bouzid, E., Kronbichler, A., ... & Gamerith, G. (2019). Hyperprogressive disease during anti-PD-1 (PDCD1)/PD-L1 (CD274) therapy: a systematic review and meta-analysis. Cancers, 11(11), 1699.

Fabrizio, F. P., Trombetta, D., Rossi, A., Sparaneo, A., Castellana, S., & Muscarella, L. A. (2018). Gene code CD274/PD-L1: from molecular basis toward cancer immunotherapy. Therapeutic advances in medical oncology, 10, 1758835918815598.

Huang, C. Y., Chiang, S. F., Ke, T. W., Chen, T. W., You, Y. S., Chen, W. T. L., & Chao, K. C. (2018). Clinical significance of programmed death 1 ligand-1 (CD274/PD-L1) and intra-tumoral CD8+ T-cell infiltration in stage II–III colorectal cancer. Scientific reports, 8(1), 1-10.

Clavé, S., Pijuan, L., Casadevall, D., Taus, Á., Gimeno, J., Hernández‐Llodrà, S., ... & Salido, M. (2018). CD 274 (PDL 1) and JAK 2 genomic amplifications in pulmonary squamous‐cell and adenocarcinoma patients. Histopathology, 72(2), 259-269.

Yang, L., Cai, Y., Zhang, D., Sun, J., Xu, C., Zhao, W., ... & Pan, C. (2018). miR-195/miR-497 regulate CD274 expression of immune regulatory ligands in triple-negative breast cancer. Journal of breast cancer, 21(4), 371-381.

Sun, J., Lian, M., Ma, H., Wang, R., Ma, Z., Wang, H., ... & Fang, J. (2018). Competing endogenous RNA network analysis of CD274, IL‑10 and FOXP3 co‑expression in laryngeal squamous cell carcinoma. Molecular medicine reports, 17(3), 3859-3869.

Lee, S. J., Jun, S. Y., Lee, I. H., Kang, B. W., Park, S. Y., Kim, H. J., ... & Kim, J. G. (2018). CD274, LAG3, and IDO1 expressions in tumor-infiltrating immune cells as prognostic biomarker for patients with MSI-high colon cancer. Journal of cancer research and clinical oncology, 144(6), 1005-1014.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus, Rhesus Recombinant CD274 protein, Fc Tag (CAT#: V2LY-0526-LY27)

Cynomolgus, Rhesus Recombinant CD274 protein, His Tag (CAT#: V2LY-0526-LY28)

Cynomolgus, Rhesus Recombinant CD274 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY29)

Canine Recombinant CD274 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY1307)

Canine Recombinant CD274 protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY1308)

Canine Recombinant CD274 protein, ECD, His Tag (CAT#: V2LY-0526-LY1309)

Canine Recombinant CD274 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY1310)

Canine Recombinant CD274 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY1311)

Cynomolgus, Rhesus Recombinant CD274 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY1733)

Cynomolgus, Rhesus Recombinant CD274 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY1734)

Antibody Pairs

CD274 Matched Antibody Pair (214) (CAT#: APMAB-214LY)

Related Products

Canine Recombinant CD274 protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY1308)

Rabbit Anti-CD274 Recombinant Antibody (CBYY-0966) (CAT#: CBMAB-0969-YY)

Human Recombinant CD274 protein, ECD, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY6082)

Mouse Recombinant CD274 protein, ECD, His Tag (CAT#: V2LY-0526-LY8759)

Cynomolgus, Rhesus Recombinant CD274 protein, His Tag (CAT#: V2LY-0526-LY28)

Human Recombinant CD274 protein, Biotin Conjugated, AVI Tag (CAT#: V2LY-0526-LY6076)

Rabbit Anti-CD274 Recombinant Antibody (
10D4) (CAT#: V2LY-0725-LY1555)

Human Recombinant CD274 protein, ECD, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY6084)

Human Recombinant CD274 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY6089)

Human Recombinant CD274 protein, ECD, Fc Tag (CAT#: V2LY-0526-LY6087)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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