Cynomolgus Recombinant CD226 protein, His Tag (V2LY-0526-LY1484)

Name: Cynomolgus Recombinant CD226 protein, His Tag
SKU: V2LY-0526-LY1484
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Cynomolgus

Tag

His Tag

Protein Construction

This product is Cynomolgus Recombinant CD226 protein, His Tag consist of Amino Acid: 1-252 and predicts a molecular mass of 28 kDa.

Molecule Mass

28 kDa

Sequence

Amino Acid: 1-252

Species

Cynomolgus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD226 Molecule

Alternative Names

CD226 Molecule; CD226 Antigen; DNAM-1; DNAM1; T Lineage-Specific Activation Antigen 1 Antigen; Platelet And T Cell Activation Antigen 1;

Function

Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell (PubMed:8673704).
Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding (PubMed:26755705).

Biological Process

Cell adhesion Source: ProtInc
Cell recognition Source: BHF-UCL
Positive regulation of Fc receptor mediated stimulatory signaling pathway Source: BHF-UCL
Positive regulation of immunoglobulin mediated immune response Source: BHF-UCL
Positive regulation of interferon-gamma production Source: GO_Central
Positive regulation of mast cell activation Source: BHF-UCL
Positive regulation of natural killer cell cytokine production Source: GO_Central
Positive regulation of natural killer cell mediated cytotoxicity Source: BHF-UCL
Positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: BHF-UCL
Positive regulation of T cell receptor signaling pathway Source: UniProtKB
Regulation of immune response Source: Reactome
Signal transduction Source: ProtInc

Cellular Location

Cell membrane

Topology

Extracellular: 19-254
Helical: 255-275
Cytoplasmic: 276-336

Bi, J. (2021). CD226: a potent driver of antitumor immunity that needs to be maintained. Cellular & Molecular Immunology, 1-2.

Feng, M., Wu, Z., Zhou, Y., Wei, Z., Tian, E., Mei, S., ... & Zhu, D. (2021). BCL9 regulates CD226 and CD96 checkpoints in CD8+ T cells to improve PD-1 response in cancer. Signal transduction and targeted therapy, 6(1), 1-14.

Huang, Z., Qi, G., Miller, J. S., & Zheng, S. G. (2020). CD226: An emerging role in immunologic diseases. Frontiers in cell and developmental biology, 8, 564.

Wang, N., Yi, H., Fang, L., Jin, J., Ma, Q., Shen, Y., ... & Chen, L. (2020). CD226 attenuates Treg proliferation via Akt and Erk signaling in an EAE model. Frontiers in immunology, 11, 1883.

Shapiro, M. R., Yeh, W. I., Longfield, J. R., Gallagher, J., Infante, C. M., Wellford, S., ... & Brusko, T. M. (2020). CD226 deletion reduces Type 1 diabetes in the NOD mouse by impairing thymocyte development and peripheral T cell activation. Frontiers in immunology, 11, 2180.

Weulersse, M., Asrir, A., Pichler, A. C., Lemaitre, L., Braun, M., Carrié, N., ... & Martinet, L. (2020). Eomes-dependent loss of the co-activating receptor CD226 restrains CD8+ T cell anti-tumor functions and limits the efficacy of cancer immunotherapy. Immunity, 53(4), 824-839.

Wang, H., Qi, J., Zhang, S., Li, Y., Tan, S., & Gao, G. F. (2019). Binding mode of the side-by-side two-IgV molecule CD226/DNAM-1 to its ligand CD155/Necl-5. Proceedings of the National Academy of Sciences, 116(3), 988-996.

Wang, N., Liang, S., Jin, J., Fang, L., Ma, Q., Wang, X., ... & Chen, L. (2019). CD226 attenuates Treg suppressive capacity via CTLA-4 and TIGIT during EAE. Immunologic research, 67(6), 486-496.

Fourcade, J., Sun, Z., Chauvin, J. M., Ka, M., Davar, D., Pagliano, O., ... & Zarour, H. M. (2018). CD226 opposes TIGIT to disrupt Tregs in melanoma. JCI insight, 3(14).

Wagner, A. K., Kadri, N., Snäll, J., Brodin, P., Gilfillan, S., Colonna, M., ... & Chambers, B. J. (2017). Expression of CD226 is associated to but not required for NK cell education. Nature communications, 8(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD226 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY3605)

Human Recombinant CD226 protein, ECD, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY3606)

Human Recombinant CD226 protein, ECD, His Tag (CAT#: V2LY-0526-LY3607)

Mouse Recombinant CD226 protein, His Tag (CAT#: V2LY-0526-LY8206)

Rat Recombinant CD226 protein, ECD, His Tag (CAT#: V2LY-0526-LY9334)

Rat Recombinant CD226 protein, hFc Tag (CAT#: V2LY-0526-LY9335)

Related Products

Mouse Recombinant CD226 protein, His Tag (CAT#: V2LY-0526-LY8206)

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Human Recombinant CD226 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY3605)

Rat Recombinant CD226 protein, hFc Tag (CAT#: V2LY-0526-LY9335)

Mouse Anti-CD226 Recombinant Antibody (CBYY-C1272) (CAT#: CBMAB-C2710-YY)

Rat Anti-CD226 Recombinant Antibody (AG14) (CAT#: V2LY-0524-LY90)

Mouse Anti-CD226 Recombinant Antibody (1X20) (CAT#: CBMAB-D0066-YC)

Mouse Anti-CD226 Recombinant Antibody (CBFYC-1169) (CAT#: CBMAB-C1227-FY)

Mouse Anti-CD226 Recombinant Antibody (D0065) (CAT#: CBMAB-D0065-YC)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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