Cynomolgus Recombinant CD276 protein, ECD, hFc Tag (V2LY-0526-LY1382)

Name: Cynomolgus Recombinant CD276 protein, ECD, hFc Tag
SKU: V2LY-0526-LY1382
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Cynomolgus

Tag

hFc Tag

Protein Construction

This product is Cynomolgus Recombinant CD276 protein, ECD, hFc Tag consist of Amino Acid: 1-465 and predicts a molecular mass of 73.8 kDa.

Molecule Mass

73.8 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-465

Species

Cynomolgus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD276 Molecule

Alternative Names

CD276 Molecule; CD276 Antigen; Costimulatory Molecule; B7 Homolog 3; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2;

Function

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

Biological Process

Positive regulation of interferon-gamma production Source: HGNC-UCL
Positive regulation of T cell proliferation Source: HGNC-UCL
Regulation of cytokine production Source: GO_Central
Regulation of immune response Source: HGNC-UCL
T cell activation Source: UniProtKB
T cell receptor signaling pathway Source: GO_Central

Cellular Location

Membrane

Topology

Extracellular: 29-466
Helical: 467-487
Cytoplasmic: 488-534

Fu, M., Zhang, J., Li, W., He, S., Zhang, J., Tennant, D., ... & Mao, Y. (2021). Gene Clusters Based on OLIG2 and CD276 Could Distinguish Molecular Profiling in Glioblastoma.

Wang, M. Y., Qi, B., Wang, F., Lin, Z. R., Li, M. Y., Yin, W. J., ... & Chen, D. P. (2021). PBK phosphorylates MSL1 to elicit epigenetic modulation of CD276 in nasopharyngeal carcinoma. Oncogenesis, 10(1), 1-14.

Liu, X., Zhelev, D., Adams, C., Chen, C., Mellors, J. W., & Dimitrov, D. S. (2021). Effective killing of cells expressing CD276 (B7-H3) by a bispecific T cell engager based on a new fully human antibody. Translational oncology, 14(12), 101232.

Kanchan, R. K., Perumal, N., Atri, P., Chirravuri Venkata, R., Thapa, I., Klinkebiel, D. L., ... & Mahapatra, S. (2020). MiR‐1253 exerts tumor‐suppressive effects in medulloblastoma via inhibition of CDK6 and CD276 (B7‐H3). Brain Pathology, 30(4), 732-745.

Li, R., Zatloukalova, P., Muller, P., Gil-Mir, M., Kote, S., Wilkinson, S., ... & Vojtesek, B. (2020). The MDM2 ligand Nutlin-3 differentially alters expression of the immune blockade receptors PD-L1 and CD276. Cellular & molecular biology letters, 25(1), 1-21.

Cheng, R., Wang, B., Cai, X. R., Chen, Z. S., Du, Q., Zhou, L. Y., ... & Chen, Y. L. (2020). CD276 promotes vasculogenic mimicry formation in hepatocellular carcinoma via the PI3K/AKT/MMPs pathway. OncoTargets and therapy, 13, 11485.

Yu, X., Wei, B., Su, R., Yao, J., Feng, X., Jiang, G., ... & Zhou, L. (2019). A risk assessment model of acute liver allograft rejection by genetic polymorphism of CD 276. Molecular genetics & genomic medicine, 7(6), e689.

Son, Y., Kwon, S. M., & Cho, J. Y. (2019). CD276 (B7‐H3) Maintains Proliferation and Regulates Differentiation in Angiogenic Function in Late Endothelial Progenitor Cells. Stem Cells, 37(3), 382-394.

Bao, R., Wang, Y., Lai, J., Zhu, H., Zhao, Y., Li, S., ... & Liu, Z. (2018). Enhancing anti-PD-1/PD-L1 immune checkpoint inhibitory cancer therapy by CD276-targeted photodynamic ablation of tumor cells and tumor vasculature. Molecular pharmaceutics, 16(1), 339-348.

Hwang, J. Y., Jeong, J. M., Kwon, M. G., Seo, J. S., Hwang, S. D., Son, M. H., ... & Park, C. I. (2018). Olive flounder CD276 (B7-H3) a coinhibitory molecule for T cells: Responses during viral hemorrhagic septicemia virus (VHSV) stimulation. Fish & shellfish immunology, 73, 228-233.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD276 protein, ECD, AF488 Conjugated, His Tag (CAT#: V2LY-0526-LY151)

Human Recombinant CD276 protein, ECD, PE Conjugated, His Tag (CAT#: V2LY-0526-LY152)

Cynomolgus Recombinant CD276 protein, ECD, His Tag (CAT#: V2LY-0526-LY1383)

Cynomolgus Recombinant CD276 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY1384)

Human Recombinant CD276 protein, APC Conjugated, His Tag (CAT#: V2LY-0526-LY2252)

Human Recombinant CD276 protein, ECD, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY2253)

Human Recombinant CD276 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2254)

Human Recombinant CD276 protein, ECD, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY2255)

Human Recombinant CD276 protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY2256)

Human Recombinant CD276 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2257)

Antibody Pairs

CD276 Matched Antibody Pair (215) (CAT#: APMAB-215LY)

Related Products

Human Recombinant CD276 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY2259)

Rat Anti-CD276 Recombinant Antibody (MJ18) (CAT#: CBMAB-C3060-CQ)

Human Recombinant CD276 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2261)

Mouse Recombinant CD276 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY7848)

Human Recombinant CD276 protein, APC Conjugated, His Tag (CAT#: V2LY-0526-LY2252)

Mouse Anti-CD276 Recombinant Antibody (CBFYC-1198) (CAT#: CBMAB-C1256-FY)

Rat Recombinant CD276 protein, His Tag (CAT#: V2LY-0526-LY9173)

Human Recombinant CD276 protein, ECD, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY2253)

Human Anti-CD276 Recombinant Antibody (
5F9) (CAT#: V2LY-0725-LY1345)

Mouse Recombinant CD276 protein, ECD, His Tag (CAT#: V2LY-0526-LY7846)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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