Cynomolgus Recombinant CD95 protein, hFc Tag (V2LY-0526-LY1510)

Name: Cynomolgus Recombinant CD95 protein, hFc Tag
SKU: V2LY-0526-LY1510
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Expressed Host

HEK293 Cells

Protein Species

Cynomolgus

Tag

hFc Tag

Protein Construction

This product is Cynomolgus Recombinant CD95 protein, hFc Tag consist of Amino Acid: 1-173 and predicts a molecular mass of 43.8 kDa.

Molecule Mass

43.8 kDa

Sequence

Amino Acid: 1-173

Species

Cynomolgus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Fas Cell Surface Death Receptor

Alternative Names

Fas Cell Surface Death Receptor; Tumor Necrosis Factor Receptor Superfamily, Member 6; Fas (TNF Receptor Superfamily, Member 6); Apoptosis-Mediating Surface Antigen FAS; TNF Receptor Superfamily Member 6; FASLG Receptor; CD95 Antigen; TNFRSF6; APT1; FAS1; Mutant Tumor Necrosis Receptor Superfamily Member 6;

Research Area

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Biological Process

Activation-induced cell death of T cells Source: GO_Central
Apoptotic process Source: MGI
Cellular response to amino acid starvation Source: CAFA
Cellular response to hyperoxia Source: UniProtKB
Cellular response to mechanical stimulus Source: UniProtKB
Extrinsic apoptotic signaling pathway Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
Fas signaling pathway Source: CAFA
Immune response Source: InterPro
Motor neuron apoptotic process Source: GO_Central
Necroptotic signaling pathway Source: BHF-UCL
Negative regulation of apoptotic process Source: GO_Central
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of apoptotic signaling pathway Source: CAFA
Positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: CAFA
Positive regulation of protein phosphorylation Source: CAFA
Protein-containing complex assembly Source: ProtInc
Regulation of apoptotic process Source: UniProtKB
Regulation of stress-activated MAPK cascade Source: CAFA
Signal transduction Source: ProtInc

Cellular Location

Isoform 1: Cell membrane; Membrane raft
Isoform 2&3&4&5&6: Secreted

Involvement in disease

Autoimmune lymphoproliferative syndrome 1A (ALPS1A):
A disorder of apoptosis that manifests in early childhood and results in the accumulation of autoreactive lymphocytes. It is characterized by non-malignant lymphadenopathy with hepatosplenomegaly, and autoimmune hemolytic anemia, thrombocytopenia and neutropenia.

Topology

Extracellular: 26-173
Helical: 174-190
Cytoplasmic: 191-335

PTM

(Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions.
Palmitoylated (PubMed:25301068). Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane (PubMed:25301068).
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD95 protein (CAT#: V2LY-0526-LY1509)

Mouse Recombinant FAS protein, His Tag (CAT#: V2LY-0526-LY8299)

Rat Recombinant FAS protein, hFc Tag (CAT#: V2LY-0526-LY9374)

Rat Recombinant FAS protein, His Tag (CAT#: V2LY-0526-LY9375)

FAS Matched Antibody Pair (410) (CAT#: APMAB-410LY)

Antibody Pairs

FAS Matched Antibody Pair (410) (CAT#: APMAB-410LY)

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Mouse Anti-FAS Recombinant Antibody (CBXF-2858) (CAT#: CBMAB-F0607-CQ)

FAS Matched Antibody Pair (410) (CAT#: APMAB-410LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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