Dog CALCA ELISA Kit (V2LY-0626-LY611)

Name: Dog CALCA ELISA Kit
SKU: V2LY-0626-LY611
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0023 ng/mL

Detection Range

0.005-1 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Dog

Assay Type

Sandwich

Reactivity

Dog

Assay Time

1.5 h

Molecule Mass

14.1 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Calcitonin Related Polypeptide Alpha

Function

Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Katacalcin is a potent plasma calcium-lowering peptide.

Biological Process

Activation of adenylate cyclase activity Source: Ensembl
Activation of protein kinase activity Source: UniProtKB
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Aging Source: Ensembl
Amyloid fibril formation Source: Reactome
Artery vasodilation involved in baroreceptor response to increased systemic arterial blood pressure Source: Ensembl
Cellular response to nerve growth factor stimulus Source: Ensembl
Cellular response to tumor necrosis factor Source: Ensembl
Detection of temperature stimulus involved in sensory perception of pain Source: Ensembl
Embryo implantation Source: UniProtKB
Feeding behavior Source: Ensembl
G protein-coupled receptor signaling pathway Source: Reactome
Inflammatory response Source: Ensembl
Monocyte chemotaxis Source: BHF-UCL
Negative regulation of bone resorption Source: UniProtKB
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Negative regulation of ossification Source: Ensembl
Negative regulation of smooth muscle contraction Source: Ensembl
Negative regulation of transcription, DNA-templated Source: UniProtKB
Neuropeptide signaling pathway Source: Ensembl
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling Pathway Source: UniProtKB
Regulation of cytosolic calcium ion concentration Source: GO_Central
Regulation of heart contraction Source: Ensembl
Response to heat Source: Ensembl
Response to pain Source: Ensembl

Cellular Location

Secreted

Appelt, J., Baranowsky, A., Jahn, D., Yorgan, T., Köhli, P., Otto, E., ... & Keller, J. (2020). The neuropeptide calcitonin gene-related peptide alpha is essential for bone healing. EBioMedicine, 59, 102970.

Sherry Wu, J., Vyas, P., Glowatzki, E., & Fuchs, P. A. (2018). Opposing expression gradients of calcitonin‐related polypeptide alpha (Calca/Cgrpα) and tyrosine hydroxylase (Th) in type II afferent neurons of the mouse cochlea. Journal of Comparative Neurology, 526(6), 1073-1073.

Wu, J. S., Vyas, P., Glowatzki, E., & Fuchs, P. A. (2018). Opposing expression gradients of calcitonin‐related polypeptide alpha (Calca/Cgrpα) and tyrosine hydroxylase (Th) in type II afferent neurons of the mouse cochlea. Journal of Comparative Neurology, 526(3), 425-438.

Alkanli, N., Sipahi, T., Ay, A., Guldiken, B., Bakir, A., Alkanli, S. S., ... & Atilgan, E. (2017). Calcitonin related polypeptide alpha gene polymorphisms according to plasma total homocysteine levels in ischemic stroke patients of Trakya Region. Biotechnology & Biotechnological Equipment, 31(6), 1184-1191.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CALCA protein, His Tag (CAT#: V2LY-0526-LY6329)

Human Recombinant CALCA protein, His Tag-1 (CAT#: V2LY-0526-LY6330)

Human Recombinant CALCA protein, Fc Tag (CAT#: V2LY-0526-LY6331)

Human CALCA ELISA Kit (CAT#: V2LY-0626-LY1319)

Mouse Calca ELISA Kit (CAT#: V2LY-0626-LY291)

Cattle CALCA ELISA Kit (CAT#: V2LY-0626-LY648)

Rat Calca ELISA Kit (CAT#: V2LY-0626-LY756)

Dog CALCA ELISA Kit (2) (CAT#: V2LY-0626-LY874)

Pig CALCA ELISA Kit (CAT#: V2LY-0626-LY905)

Pig CALCA ELISA Kit (2) (CAT#: V2LY-0626-LY938)

Related Products

Human CALCA ELISA Kit (CAT#: V2LY-0626-LY1319)

Cattle CALCA ELISA Kit (CAT#: V2LY-0626-LY648)

Mouse Anti-CALCA Recombinant Antibody (02) (CAT#: CBMAB-C1650-LY)

Mouse Anti-CALCA Recombinant Antibody (CBYC-A923) (CAT#: CBMAB-A4267-YC)

Human Recombinant CALCA protein, His Tag-1 (CAT#: V2LY-0526-LY6330)

Pig CALCA ELISA Kit (CAT#: V2LY-0626-LY905)

Mouse Anti-CALCA Recombinant Antibody (CBFYC-0731) (CAT#: CBMAB-C0786-FY)

Dog CALCA ELISA Kit (2) (CAT#: V2LY-0626-LY874)

Mouse Anti-CALCA Recombinant Antibody (CBFYC-0475) (CAT#: CBMAB-C0522-FY)

Mouse Anti-CALCA Recombinant Antibody (CBFYC-0327) (CAT#: CBMAB-C0363-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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