Human BMP1 ELISA Kit (V2LY-0626-LY2612)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0027 ng/mL
Detection Range
0.005-1 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
111.2 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Bone Morphogenetic Protein 1
Function
Metalloprotease that plays key roles in regulating the formation of the extracellular matrix (ECM) via processing of various precursor proteins into mature functional enzymes or structural proteins (PubMed:33206546).
Thereby participates in several developmental and physiological processes such as cartilage and bone formation, muscle growth and homeostasis, wound healing and tissue repair (PubMed:33169406, PubMed:32636307).
Roles in ECM formation include cleavage of the C-terminal propeptides from procollagens such as procollagen I, II and III or the proteolytic activation of the enzyme lysyl oxidase LOX, necessary to formation of covalent cross-links in collagen and elastic fibers (PubMed:31152061, PubMed:33206546).
Additional substrates include matricellular thrombospondin-1/THBS1 whose cleavage leads to cell adhesion disruption and TGF-beta activation (PubMed:32636307).
Isoform BMP1-3: Plays an important role in bone repair by acting as a coactivator of BMP7.
Biological Process
Cartilage condensation Source: ProtInc
Cell differentiation Source: UniProtKB-KW
Collagen fibril organization Source: Reactome
Extracellular matrix disassembly Source: Reactome
High-density lipoprotein particle assembly Source: Reactome
Multicellular organism development Source: ProtInc
Ossification Source: UniProtKB-KW
Positive regulation of cartilage development Source: MGI
Proteolysis Source: UniProtKB
Skeletal system development Source: ProtInc
Cellular Location
Trans-Golgi network; Extracellular matrix; Secreted. Co-localizes with POSTN in the Golgi.
Isoform BMP1-3: Secreted
Involvement in disease
Osteogenesis imperfecta 13 (OI13): An autosomal recessive form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI13 is characterized by normal teeth, faint blue sclerae, severe growth deficiency, borderline osteoporosis, severe bone deformity, and recurrent fractures affecting both upper and lower limbs.
PTM
Proteolytically activated in the trans-Golgi network by furin-like/paired basic proprotein convertases, cleavage is not required for secretion.

Rafi, J. H., Jafar, T., Pathan, M. T., Reza, R., Islam, S., Sourna, I. J., ... & Ahammad, F. (2021). High expression of bone morphogenetic protein 1 (BMP1) is associated with a poor survival rate in human gastric cancer, a dataset approaches. Genomics, 113(1), 1141-1154.

Wang, J., Xie, X., Muench, N. A., Massoudi, D., Xu, C., Greenspan, D. S., & Feng, J. Q. (2021). Proteinase bone morphogenetic protein 1, but not tolloid‐like 1, plays a dominant role in maintaining periodontal homeostasis. Journal of Periodontology, 92(7), 1018-1029.

Strøm, T. B., Bjune, K., & Leren, T. P. (2020). Bone morphogenetic protein 1 cleaves the linker region between ligand-binding repeats 4 and 5 of the LDL receptor and makes the LDL receptor non-functional. Human molecular genetics, 29(8), 1229-1238.

Eshed-Eisenbach, Y., Devaux, J., Vainshtein, A., Golani, O., Lee, S. J., Feinberg, K., ... & Peles, E. (2020). Precise spatiotemporal control of nodal Na+ channel clustering by bone morphogenetic protein-1/Tolloid-like proteinases. Neuron, 106(5), 806-815.

Su, Z., He, L., Shang, H., Dai, T., Xu, F., & Zhao, J. (2020). Overexpression of Bone Morphogenetic Protein-1 Promotes Osteogenesis of Bone Marrow Mesenchymal Stem Cells In Vitro. Medical science monitor: international medical journal of experimental and clinical research, 26, e920122-1.

Banerjee, S., Andrew, R. J., Duff, C. J., Fisher, K., Jackson, C. D., Lawrence, C. B., ... & Hooper, N. M. (2019). Proteolysis of the low density lipoprotein receptor by bone morphogenetic protein-1 regulates cellular cholesterol uptake. Scientific reports, 9(1), 1-14.

Rosell-Garcia, T., & Rodríguez-Pascual, F. (2018). Enhancement of collagen deposition and cross-linking by coupling lysyl oxidase with bone morphogenetic protein-1 and its application in tissue engineering. Scientific reports, 8(1), 1-12.

Kallander, L. S., Washburn, D., Hilfiker, M. A., Eidam, H. S., Lawhorn, B. G., Prendergast, J., ... & Willette, B. (2018). Reverse hydroxamate inhibitors of bone morphogenetic protein 1. ACS medicinal chemistry letters, 9(7), 736-740.

Hsieh, Y. Y., Tung, S. Y., Pan, H. Y., Yen, C. W., Xu, H. W., Deng, Y. F., ... & Li, C. (2018). Upregulation of bone morphogenetic protein 1 is associated with poor prognosis of late-stage gastric Cancer patients. BMC cancer, 18(1), 1-13.

Zhang, H., Jani, P., Liang, T., Lu, Y., & Qin, C. (2017). Inactivation of bone morphogenetic protein 1 (Bmp1) and tolloid-like 1 (Tll1) in cells expressing type I collagen leads to dental and periodontal defects in mice. Journal of molecular histology, 48(2), 83-98.

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For research use only. Not intended for any clinical use.

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