Human BMP15 ELISA Kit (V2LY-0626-LY2618)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0052 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
45.1 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Bone Morphogenetic Protein 15
Function
May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth.
Biological Process
BMP signaling pathway Source: GO_Central
Cellular protein metabolic process Source: Reactome
Female gamete generation Source: ProtInc
Granulosa cell development Source: InterPro
Ovarian follicle development Source: InterPro
Positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
Positive regulation of transcription, DNA-templated Source: Ensembl
Post-translational protein modification Source: Reactome
SMAD protein signal transduction Source: GO_Central
Cellular Location
Secreted
Involvement in disease
Ovarian dysgenesis 2 (ODG2): A disorder characterized by lack of spontaneous pubertal development, primary amenorrhea, uterine hypoplasia, and hypergonadotropic hypogonadism as a result of streak gonads.
Premature ovarian failure 4 (POF4): An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

Flossmann, G., Wurmser, C., Pausch, H., Tenghe, A., Dodenhoff, J., Dahinten, G., ... & Fries, R. (2021). A nonsense mutation of bone morphogenetic protein-15 (BMP15) causes both infertility and increased litter size in pigs. BMC genomics, 22(1), 1-9.

Najafabadi, H. A., Khansefid, M., Mahmoud, G. G., Haruna, I. L., Zhou, H., & Hickford, J. G. (2021). Identification of sequence variation in the oocyte-derived bone morphogenetic protein 15 (BMP15) gene (BMP15) associated with litter size in New Zealand sheep (Ovis aries) breeds. Molecular Biology Reports, 1-8.

Faiza, H., Khan, M., Rafiq, M., Khan, A. A., Rind, N. A., & Naqvi, S. H. A. (2021). Two novel mutations in exon 2 of bone morphogenetic protein (BMP) 15 gene in Pakistani infertile females. Saudi Journal of Biological Sciences, 28(9), 5364-5370.

Taheri, M., Saki, G., Nikbakht, R., & Eftekhari, A. R. (2021). Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cells derived from human follicular fluid to oocyte‐like cell. Cell Biology International, 45(1), 127-139.

Yu, H., Wang, Y., Wang, M., Liu, Y., Cheng, J., & Zhang, Q. (2020). Growth differentiation factor 9 (gdf9) and bone morphogenetic protein 15 (bmp15) are potential intraovarian regulators of steroidogenesis in Japanese flounder (Paralichthys olivaceus). General and Comparative Endocrinology, 297, 113547.

Coelho, L. A., do Carmo Buonfiglio, D., Kuwabara, W. M. T., Andrade-Silva, J., Gomes, P. R. L., Scialfa, J. H., ... & Cipolla-Neto, J. (2020). Melatonin regulates the expression of Bone Morphogenetic Protein 15 (Bmp-15), Growth Differentiation Factor 9 (Gdf-9) and LH receptor (Lhr) genes in developing follicles of rats. Melatonin Research, 3(4), 515-533.

Tang, T., Lin, Q., Qin, Y., Liang, X., Guo, Y., Cong, P., ... & He, Z. (2020). Effects of bone morphogenetic protein 15 (BMP15) knockdown on porcine testis morphology and spermatogenesis. Reproduction, Fertility and Development, 32(11), 999-1011.

Garcia, P., Aspee, K., Ramirez, G., Dettleff, P., Palomino, J., Peralta, O. A., ... & De los Reyes, M. (2019). Influence of growth differentiation factor 9 and bone morphogenetic protein 15 on in vitro maturation of canine oocytes. Reproduction in Domestic Animals, 54(2), 373-380.

Qin, Y., Tang, T., Li, W., Liu, Z., Yang, X., Shi, X., ... & He, Z. (2019). Bone morphogenetic protein 15 knockdown inhibits porcine ovarian follicular development and ovulation. Frontiers in cell and developmental biology, 7, 286.

Nagdy, H., Mahmoud, K. G. M., Kandiel, M. M., Helmy, N. A., Ibrahim, S. S., Nawito, M. F., & Othman, O. E. (2018). PCR-RFLP of bone morphogenetic protein 15 (BMP15/FecX) gene as a candidate for prolificacy in sheep. International journal of veterinary science and medicine, 6(sup1), S68-S72.

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For research use only. Not intended for any clinical use.

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