Mouse Adam33 ELISA Kit (V2LY-0626-LY2318)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0057 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
87.0 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
ADAM Metallopeptidase Domain 33
Biological Process
Proteolysis
Cellular Location
Membrane
Involvement in disease
Asthma (ASTHMA): The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with wheezing due to spasmodic contraction of the bronchi.
Topology
Extracellular: 30-701 aa
Helical: 702-722 aa
Cytoplasmic: 723-813 aa
PTM
The precursor is cleaved by a furin endopeptidase.

Mamoor, S. (2021). Differential expression of ADAM33 in cancers of the breast.

Zihlif, M., Imraish, A., Al-Rawashdeh, B., Qteish, A., Husami, R., Husami, R., ... & Lee, S. J. (2021). The Association of IgE Levels with ADAM33 Genetic Polymorphisms among Asthmatic Patients. Journal of personalized medicine, 11(5), 329.

Yan, F., Hao, Y., Gong, X., Sun, H., Ding, J., & Wang, J. (2021). Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling. Molecular Medicine Reports, 24(1), 1-12.

Ghani, M. U., Sabar, M. F., Iqbal Bano, M. S., Akram, M., Ifrah Khalid, A. M., & Khan, M. U. (2019). Evaluation of ADAM33 gene’s single nucleotide polymorphism variants against asthma and the unique pattern of inheritance in Northern and Central Punjab, Pakistan. Saudi medical journal, 40(8), 774.

Li, H. F., Yan, L. P., Wang, K., Li, X. T., Liu, H. X., & Tan, W. (2019). Association between ADAM33 polymorphisms and asthma risk: a systematic review and meta-analysis. Respiratory research, 20(1), 1-18.

Ghaemi, M. R., Hemmati, S., Rezaei, A., Sadr, M., Mohebbi, B., Ghaffaripour, H., ... & Mahdaviani, S. A. (2019). Association of ADAM33 T1 Polymorphism With Subgroups of Pediatric Asthma Patients in Iran. Acta Medica Iranica, 635-639.

Fang, L., Wu, J., Huang, T., Zhang, P., Xin, X., & Shi, Y. (2018). TGF-β1 stimulates epithelial‑mesenchymal transition mediated by ADAM33. Experimental and therapeutic medicine, 15(1), 985-992.

Yang, P. J., Hou, M. F., Tsai, E. M., Liang, S. S., Chiu, C. C., Ou‑Yang, F., ... & Wang, T. N. (2018). Breast cancer is associated with methylation and expression of the a disintegrin and metalloproteinase domain 33 (ADAM33) gene affected by endocrine‑disrupting chemicals. Oncology reports, 40(5), 2766-2777.

Sun, F. J., Zou, L. Y., Tong, D. M., Lu, X. Y., Li, J., & Deng, C. B. (2017). Association between ADAM metallopeptidase domain 33 gene polymorphism and risk of childhood asthma: a meta-analysis. Brazilian Journal of Medical and Biological Research, 50.

Deng, R., Zhao, F., & Zhong, X. (2017). T1 polymorphism in a disintegrin and metalloproteinase 33 (ADAM33) gene may contribute to the risk of childhood asthma in Asians. Inflammation Research, 66(5).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rat Anti-ADAM33 Recombinant Antibody (V2-179792) (CAT#: CBMAB-A1154-YC)

Mouse Anti-ADAM33 Recombinant Antibody (V2-179794) (CAT#: CBMAB-A1156-YC)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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