Human CD7 ELISA Kit (V2LY-0626-LY5307)

Name: Human CD7 ELISA Kit
SKU: V2LY-0626-LY5307
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.032 ng/mL

Detection Range

0.05-18 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

25.4 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

CD7 Molecule

Alternative Names

CD7 Molecule; T-Cell Leukemia Antigen; T-Cell Surface Antigen Leu-9; T-Cell Antigen CD7; CD7 Antigen (P41); P41 Protein;

Biological Process

Adaptive immune response Source: UniProtKB-KW
Immune response Source: UniProtKB
T cell activation Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: UniProtKB

Cellular Location

Membrane

Topology

Extracellular: 26-180
Helical: 181-201
Cytoplasmic: 202-240

Li, S., Wang, X., Yuan, Z., Liu, L., Luo, L., Li, Y., ... & Wang, S. (2021). Eradication of T-ALL Cells by CD7-targeted Universal CAR-T Cells and Initial Test of Ruxolitinib-based CRS Management. Clinical Cancer Research, 27(5), 1242-1246.

Kim, M. Y., Cooper, M. L., Jacobs, M. T., Ritchey, J. K., Hollaway, J., Fehniger, T. A., & DiPersio, J. F. (2021). CD7-deleted hematopoietic stem cells can restore immunity after CAR T cell therapy. JCI insight, 6(16).

Zhang, J., Jain, A., Milhas, S., Williamson, D. J., Mysliwy, J., Lodge, A., ... & Rabbitts, T. H. (2021). An antibody-drug conjugate with intracellular drug release properties showing specific cytotoxicity against CD7-positive cells. Leukemia Research, 108, 106626.

Gomes-Silva, D., Atilla, E., Atilla, P. A., Mo, F., Tashiro, H., Srinivasan, M., ... & Mamonkin, M. (2019). CD7 CAR T cells for the therapy of acute myeloid leukemia. Molecular Therapy, 27(1), 272-280.

You, F., Wang, Y., Jiang, L., Zhu, X., Chen, D., Yuan, L., ... & Yang, L. (2019). A novel CD7 chimeric antigen receptor-modified NK-92MI cell line targeting T-cell acute lymphoblastic leukemia. American journal of cancer research, 9(1), 64.

Mayer, K. E., Mall, S., Yusufi, N., Gosmann, D., Steiger, K., Russelli, L., ... & Krackhardt, A. M. (2018). T-cell functionality testing is highly relevant to developing novel immuno-tracers monitoring T cells in the context of immunotherapies and revealed CD7 as an attractive target. Theranostics, 8(21), 6070.

Gomes-Silva, D., Srinivasan, M., Sharma, S., Lee, C. M., Wagner, D. L., Davis, T. H., ... & Mamonkin, M. (2017). CD7-edited T cells expressing a CD7-specific CAR for the therapy of T-cell malignancies. Blood, The Journal of the American Society of Hematology, 130(3), 285-296.

Yu, Y., Li, J., Zhu, X., Tang, X., Bao, Y., Sun, X., ... & Yang, L. (2017). Humanized CD7 nanobody-based immunotoxins exhibit promising anti-T-cell acute lymphoblastic leukemia potential. International journal of nanomedicine, 12, 1969.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD7 protein, ECD, His Tag (CAT#: V2LY-0526-LY1448)

Human Recombinant CD7 protein, AF488 Conjugated, His Tag (CAT#: V2LY-0526-LY2922)

Human Recombinant CD7 protein, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY2923)

Human Recombinant CD7 protein, APC Conjugated, His Tag (CAT#: V2LY-0526-LY2924)

Human Recombinant CD7 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2925)

Human Recombinant CD7 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2926)

Human Recombinant CD7 protein, His Tag (CAT#: V2LY-0526-LY2927)

Human Recombinant CD7 protein, PE Conjugated, His Tag (CAT#: V2LY-0526-LY2928)

Mouse Recombinant CD7 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8059)

Mouse Recombinant CD7 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY8060)

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Human Recombinant CD7 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2926)

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Human Recombinant CD7 protein, PE Conjugated, His Tag (CAT#: V2LY-0526-LY2928)

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Rat Recombinant CD7 protein, hFc Tag (CAT#: V2LY-0526-LY9265)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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