Human CRBN ELISA Kit (V2LY-0626-LY3510)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.043 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
50.5 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Cereblon
Function
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 (Probable). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed:20223979, PubMed:24328678, PubMed:25043012, PubMed:25108355).

Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons (PubMed:18414909, PubMed:29530986).

Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1 (PubMed:18414909).

May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism (By similarity).
Biological Process
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm; Membrane
Involvement in disease
Mental retardation, autosomal recessive 2A (MRT2A):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features.
PTM
Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.

Kim, H. K., Seol, J. E., Ahn, S. W., Jeon, S., Park, C. S., & Han, J. (2021). Cereblon: promise and challenges for combating human diseases. Pflügers Archiv-European Journal of Physiology, 473(11), 1695-1711.

Ito, T., Yamaguchi, Y., & Handa, H. (2021). Exploiting ubiquitin ligase cereblon as a target for small-molecule compounds in medicine and chemical biology. Cell Chemical Biology, 28(7), 987-999.

Jones, J. R., Barber, A., Le Bihan, Y. V., Weinhold, N., Ashby, C., Walker, B. A., ... & Pawlyn, C. (2021). Mutations in CRBN and other cereblon pathway genes are infrequently associated with acquired resistance to immunomodulatory drugs. Leukemia, 35(10), 3017-3020.

Powell, C. E., Du, G., Che, J., He, Z., Donovan, K. A., Yue, H., ... & Gray, N. S. (2020). Selective degradation of GSPT1 by cereblon modulators identified via a focused combinatorial library. ACS chemical biology, 15(10), 2722-2730.

Bjorklund, C. C., Kang, J., Amatangelo, M., Polonskaia, A., Katz, M., Chiu, H., ... & Thakurta, A. (2020). Iberdomide (CC-220) is a potent cereblon E3 ligase modulator with antitumor and immunostimulatory activities in lenalidomide-and pomalidomide-resistant multiple myeloma cells with dysregulated CRBN. Leukemia, 34(4), 1197-1201.

Li, L., Xue, W., Shen, Z., Liu, J., Hu, M., Cheng, Z., ... & Zhao, J. (2020). A cereblon modulator CC-885 induces CRBN-and p97-dependent PLK1 degradation and synergizes with volasertib to suppress lung cancer. Molecular Therapy-Oncolytics, 18, 215-225.

Yang, S. J., Jeon, S. J., Van Nguyen, T., Deshaies, R. J., Park, C. S., & Lee, K. M. (2020). Ubiquitin-dependent proteasomal degradation of AMPK gamma subunit by Cereblon inhibits AMPK activity. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1867(8), 118729.

Asatsuma-Okumura, T., Ito, T., & Handa, H. (2019). Molecular mechanisms of cereblon-based drugs. Pharmacology & Therapeutics, 202, 132-139.

Dimopoulos, K., Fibiger Munch-Petersen, H., Winther Eskelund, C., Dissing Sjö, L., Ralfkiaer, E., Gimsing, P., & Grønbaek, K. (2019). Expression of CRBN, IKZF1, and IKZF3 does not predict lenalidomide sensitivity and mutations in the cereblon pathway are infrequent in multiple myeloma. Leukemia & Lymphoma, 60(1), 180-188.

Steinebach, C., Lindner, S., Udeshi, N. D., Mani, D. C., Kehm, H., Köpff, S., ... & Krönke, J. (2018). Homo-PROTACs for the chemical knockdown of cereblon. ACS chemical biology, 13(9), 2771-2782.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CRBN protein, GST Tag (CAT#: V2LY-0526-LY3362)

Human Recombinant CRBN & DDB1 protein, Hetero Dimer , GST Tag (CAT#: V2LY-0526-LY3359)

Human Recombinant CRBN & DDB1 protein, Hetero Dimer , His Tag (CAT#: V2LY-0526-LY3360)

Human Recombinant CRBN & DDB1 & CUL4A & RBX1 protein, His Tag (CAT#: V2LY-0526-LY3358)

Human Recombinant CRBN & DDB1 protein, Dimer, His & Flag Tag (CAT#: V2LY-0526-LY3361)

Related Products

Human Recombinant CRBN & DDB1 protein, Dimer, His & Flag Tag (CAT#: V2LY-0526-LY3361)

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Human Recombinant CRBN protein, GST Tag (CAT#: V2LY-0526-LY3362)

Mouse Anti-CRBN Recombinant Antibody (C5699) (CAT#: CBMAB-C5699-LY)

Mouse Anti-CRBN Recombinant Antibody (CBYY-C2841) (CAT#: CBMAB-C4282-YY)

Rabbit Anti-CRBN Recombinant Antibody (CP0367) (CAT#: CBMAB-CP0367-LY)

Human Recombinant CRBN & DDB1 & CUL4A & RBX1 protein, His Tag (CAT#: V2LY-0526-LY3358)

Human Recombinant CRBN & DDB1 protein, Hetero Dimer , His Tag (CAT#: V2LY-0526-LY3360)

Human Recombinant CRBN & DDB1 protein, Hetero Dimer , GST Tag (CAT#: V2LY-0526-LY3359)

Isotype control

For research use only. Not intended for any clinical use.

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