Mouse Anti-CRBN Recombinant Antibody (CBYY-C2841) (CBMAB-C4282-YY)

Name: Mouse Anti-CRBN Recombinant Antibody (CBYY-C2841)
SKU: CBMAB-C4282-YY
Rating: 5 (1 reviews)

See all CRBN antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C2841

Application

IHC, IF

Immunogen

Synthetic peptide Human CRBN.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	5-25 µg/ml
IHC	1-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Concentration

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cereblon

Entrez Gene ID

51185

UniProt ID

Q96SW2

Alternative Names

C-Reactive Protein; C-Reactive Protein, Pentraxin-Related; Pentraxin 1; PTX1;

Function

Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 (Probable). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed:20223979, PubMed:24328678, PubMed:25043012, PubMed:25108355).

Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons (PubMed:18414909, PubMed:29530986).

Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1 (PubMed:18414909).

May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism (By similarity).

Biological Process

Proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
Protein ubiquitination Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm; Membrane

Involvement in disease

Mental retardation, autosomal recessive 2A (MRT2A):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features.

PTM

Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.

Kim, H. K., Seol, J. E., Ahn, S. W., Jeon, S., Park, C. S., & Han, J. (2021). Cereblon: promise and challenges for combating human diseases. Pflügers Archiv-European Journal of Physiology, 473(11), 1695-1711.

Ito, T., Yamaguchi, Y., & Handa, H. (2021). Exploiting ubiquitin ligase cereblon as a target for small-molecule compounds in medicine and chemical biology. Cell Chemical Biology, 28(7), 987-999.

Jones, J. R., Barber, A., Le Bihan, Y. V., Weinhold, N., Ashby, C., Walker, B. A., ... & Pawlyn, C. (2021). Mutations in CRBN and other cereblon pathway genes are infrequently associated with acquired resistance to immunomodulatory drugs. Leukemia, 35(10), 3017-3020.

Powell, C. E., Du, G., Che, J., He, Z., Donovan, K. A., Yue, H., ... & Gray, N. S. (2020). Selective degradation of GSPT1 by cereblon modulators identified via a focused combinatorial library. ACS chemical biology, 15(10), 2722-2730.

Bjorklund, C. C., Kang, J., Amatangelo, M., Polonskaia, A., Katz, M., Chiu, H., ... & Thakurta, A. (2020). Iberdomide (CC-220) is a potent cereblon E3 ligase modulator with antitumor and immunostimulatory activities in lenalidomide-and pomalidomide-resistant multiple myeloma cells with dysregulated CRBN. Leukemia, 34(4), 1197-1201.

Li, L., Xue, W., Shen, Z., Liu, J., Hu, M., Cheng, Z., ... & Zhao, J. (2020). A cereblon modulator CC-885 induces CRBN-and p97-dependent PLK1 degradation and synergizes with volasertib to suppress lung cancer. Molecular Therapy-Oncolytics, 18, 215-225.

Yang, S. J., Jeon, S. J., Van Nguyen, T., Deshaies, R. J., Park, C. S., & Lee, K. M. (2020). Ubiquitin-dependent proteasomal degradation of AMPK gamma subunit by Cereblon inhibits AMPK activity. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1867(8), 118729.

Asatsuma-Okumura, T., Ito, T., & Handa, H. (2019). Molecular mechanisms of cereblon-based drugs. Pharmacology & Therapeutics, 202, 132-139.

Dimopoulos, K., Fibiger Munch-Petersen, H., Winther Eskelund, C., Dissing Sjö, L., Ralfkiaer, E., Gimsing, P., & Grønbaek, K. (2019). Expression of CRBN, IKZF1, and IKZF3 does not predict lenalidomide sensitivity and mutations in the cereblon pathway are infrequent in multiple myeloma. Leukemia & Lymphoma, 60(1), 180-188.

Steinebach, C., Lindner, S., Udeshi, N. D., Mani, D. C., Kehm, H., Köpff, S., ... & Krönke, J. (2018). Homo-PROTACs for the chemical knockdown of cereblon. ACS chemical biology, 13(9), 2771-2782.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CRBN Recombinant Antibody (CBYY-C2841)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Rabbit Anti-CRBN Recombinant Antibody (CP0367) (CAT#: CBMAB-CP0367-LY)

Mouse Anti-CRBN Recombinant Antibody (C5699) (CAT#: CBMAB-C5699-LY)

Rabbit Anti-CRBN Recombinant Antibody (AP1688) (CAT#: V2LY-1206-LY1080)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry