Human CRISP1 ELISA Kit (V2LY-0626-LY4464)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
5.38 ng/mL
Detection Range
10-2000 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
28.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Cysteine Rich Secretory Protein 1
Function
May have a role in sperm-egg fusion and maturation.
Biological Process
Binding of sperm to zona pellucida Source: GO_Central
Fusion of sperm to egg plasma membrane involved in single fertilization Source: ProtInc

Kumar, S., Leeb, A. S., Vaughan, A. M., & Kappe, S. H. (2022). A Plasmodium falciparum Cysteine Rich Secretory protein uniquely localizes to one end of male gametes. Molecular and Biochemical Parasitology, 111447.

Jorasia, K., Paul, R. K., Rathore, N. S., Lal, P., Singh, R., & Sareen, M. (2021). Production of bioactive recombinant ovine cysteine-rich secretory protein 1 in Escherichia coli. Systems biology in reproductive medicine, 67(6), 471-481.

Arévalo, L., Brukman, N. G., Cuasnicú, P. S., & Roldan, E. R. (2020). Evolutionary analysis of genes coding for Cysteine-RIch Secretory Proteins (CRISPs) in mammals. BMC evolutionary biology, 20(1), 1-13.

Bernardes, C. P., Menaldo, D. L., Zoccal, K. F., Boldrini-Franca, J., Peigneur, S., Arantes, E. C., ... & Sampaio, S. V. (2019). First report on BaltCRP, a cysteine-rich secretory protein (CRISP) from Bothrops alternatus venom: effects on potassium channels and inflammatory processes. International journal of biological macromolecules, 140, 556-567.

Weigel Muñoz, M., Battistone, M. A., Carvajal, G., Maldera, J. A., Curci, L., Torres, P., ... & Cuasnicú, P. S. (2018). Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-Rich Secretory Protein 1 (CRISP1). Biology of Reproduction, 99(2), 373-383.

Lodovicho, M. E., Costa, T. R., Bernardes, C. P., Menaldo, D. L., Zoccal, K. F., Carone, S. E., ... & Sampaio, S. V. (2017). Investigating possible biological targets of Bj-CRP, the first cysteine-rich secretory protein (CRISP) isolated from Bothrops jararaca snake venom. Toxicology letters, 265, 156-169.

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For research use only. Not intended for any clinical use.

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