Mouse Recombinant CRISP1 protein, His Tag (V2LY-0526-LY8163)

Name: Mouse Recombinant CRISP1 protein, His Tag
SKU: V2LY-0526-LY8163
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant CRISP1 protein, His Tag consist of Amino Acid: 1-244 and predicts a molecular mass of 27.1 kDa.

Molecule Mass

27.1 kDa

Sequence

Amino Acid: 1-244

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cysteine Rich Secretory Protein 1

Function

May have a role in sperm-egg fusion and maturation.

Biological Process

Binding of sperm to zona pellucida Source: GO_Central
Fusion of sperm to egg plasma membrane involved in single fertilization Source: ProtInc

Kumar, S., Leeb, A. S., Vaughan, A. M., & Kappe, S. H. (2022). A Plasmodium falciparum Cysteine Rich Secretory protein uniquely localizes to one end of male gametes. Molecular and Biochemical Parasitology, 111447.

Jorasia, K., Paul, R. K., Rathore, N. S., Lal, P., Singh, R., & Sareen, M. (2021). Production of bioactive recombinant ovine cysteine-rich secretory protein 1 in Escherichia coli. Systems biology in reproductive medicine, 67(6), 471-481.

Arévalo, L., Brukman, N. G., Cuasnicú, P. S., & Roldan, E. R. (2020). Evolutionary analysis of genes coding for Cysteine-RIch Secretory Proteins (CRISPs) in mammals. BMC evolutionary biology, 20(1), 1-13.

Bernardes, C. P., Menaldo, D. L., Zoccal, K. F., Boldrini-Franca, J., Peigneur, S., Arantes, E. C., ... & Sampaio, S. V. (2019). First report on BaltCRP, a cysteine-rich secretory protein (CRISP) from Bothrops alternatus venom: effects on potassium channels and inflammatory processes. International journal of biological macromolecules, 140, 556-567.

Weigel Muñoz, M., Battistone, M. A., Carvajal, G., Maldera, J. A., Curci, L., Torres, P., ... & Cuasnicú, P. S. (2018). Influence of the genetic background on the reproductive phenotype of mice lacking Cysteine-Rich Secretory Protein 1 (CRISP1). Biology of Reproduction, 99(2), 373-383.

Lodovicho, M. E., Costa, T. R., Bernardes, C. P., Menaldo, D. L., Zoccal, K. F., Carone, S. E., ... & Sampaio, S. V. (2017). Investigating possible biological targets of Bj-CRP, the first cysteine-rich secretory protein (CRISP) isolated from Bothrops jararaca snake venom. Toxicology letters, 265, 156-169.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CRISP1 protein, hFc Tag (CAT#: V2LY-0526-LY8162)

Related Products

Rat Anti-CRISP1 Recombinant Antibody (CBYY-C0678) (CAT#: CBMAB-C2001-YY)

Mouse Recombinant CRISP1 protein, hFc Tag (CAT#: V2LY-0526-LY8162)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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