Human CST7 ELISA Kit (V2LY-0626-LY3453)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.12 ng/mL
Detection Range
0.3-90 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
16.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Cystatin F
Function
Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
Biological Process
Immune response Source: ProtInc
Inhibition of cysteine-type endopeptidase activity Source: GO_Central
Negative regulation of microglial cell activation Source: GO_Central
Negative regulation of peptidase activity Source: ARUK-UCL
Positive regulation of myelination Source: GO_Central
Cellular Location
Secreted; Cytoplasm

Sawyer, A. J., Garand, M., Chaussabel, D., & Feng, C. G. (2021). Transcriptomic Profiling Identifies Neutrophil-Specific Upregulation of Cystatin F as a Marker of Acute Inflammation in Humans. Frontiers in immunology, 12.

Senjor, E., Perišić Nanut, M., Breznik, B., Mitrović, A., Mlakar, J., Rotter, A., ... & Kos, J. (2021). Cystatin F acts as a mediator of immune suppression in glioblastoma. Cellular Oncology, 44(5), 1051-1063.

Yang, C., Yu, T., Liu, Z., Ye, X., Liao, X., Wang, X., ... & Peng, T. (2019). Cystatin F as a key family 2 cystatin subunit and prognostic biomarker for early‑stage pancreatic ductal adenocarcinoma. Oncology reports, 42(1), 79-90.

Dautović, E., Nanut, M. P., Softić, A., & Kos, J. (2018). The transcription factor C/EBP α controls the role of cystatin F during the differentiation of monocytes to macrophages. European Journal of Cell Biology, 97(7), 463-473.

Kos, J., Nanut, M. P., Prunk, M., Sabotič, J., Dautović, E., & Jewett, A. (2018). Cystatin F as a regulator of immune cell cytotoxicity. Cancer Immunology, Immunotherapy, 67(12), 1931-1938.

Nuvolone, M., Schmid, N., Miele, G., Sorce, S., Moos, R., Schori, C., ... & Aguzzi, A. (2017). Cystatin F is a biomarker of prion pathogenesis in mice. PLoS One, 12(2), e0171923.

Perišić Nanut, M., Sabotič, J., Švajger, U., Jewett, A., & Kos, J. (2017). Cystatin F affects natural killer cell cytotoxicity. Frontiers in immunology, 8, 1459.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CST7 protein (CAT#: V2LY-0526-LY3478)

Human Recombinant CST7 protein, hFc Tag (CAT#: V2LY-0526-LY3479)

Human Recombinant CST7 protein, His Tag (CAT#: V2LY-0526-LY3480)

Mouse Recombinant CST7 protein, hFc Tag (CAT#: V2LY-0526-LY8186)

Mouse Cst7 ELISA Kit (CAT#: V2LY-0626-LY2255)

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Human Recombinant CST7 protein (CAT#: V2LY-0526-LY3478)

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Human Recombinant CST7 protein, hFc Tag (CAT#: V2LY-0526-LY3479)

Mouse Anti-CST7 Recombinant Antibody (CBCNC-431) (CAT#: V2LY-0125-LY152)

Human Recombinant CST7 protein, His Tag (CAT#: V2LY-0526-LY3480)

Mouse Anti-CST7 Recombinant Antibody (CBFYC-2348) (CAT#: V2LY-0125-LY151)

Mouse Anti-CST7 Recombinant Antibody (C5060) (CAT#: V2LY-0125-LY150)

Mouse Recombinant CST7 protein, hFc Tag (CAT#: V2LY-0526-LY8186)

Mouse Cst7 ELISA Kit (CAT#: V2LY-0626-LY2255)

Mouse Anti-CST7 Recombinant Antibody (C5040) (CAT#: V2LY-0125-LY149)

Isotype control

For research use only. Not intended for any clinical use.

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