Mouse Recombinant CST7 protein, hFc Tag (V2LY-0526-LY8186)

Name: Mouse Recombinant CST7 protein, hFc Tag
SKU: V2LY-0526-LY8186
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

hFc Tag

Protein Construction

This product is Mouse Recombinant CST7 protein, hFc Tag consist of Amino Acid: 1-144 and predicts a molecular mass of 41.4 kDa.

Molecule Mass

41.4 kDa

Sequence

Amino Acid: 1-144

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>93% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cystatin F

Function

Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.

Biological Process

Immune response Source: ProtInc
Inhibition of cysteine-type endopeptidase activity Source: GO_Central
Negative regulation of microglial cell activation Source: GO_Central
Negative regulation of peptidase activity Source: ARUK-UCL
Positive regulation of myelination Source: GO_Central

Cellular Location

Secreted; Cytoplasm

Sawyer, A. J., Garand, M., Chaussabel, D., & Feng, C. G. (2021). Transcriptomic Profiling Identifies Neutrophil-Specific Upregulation of Cystatin F as a Marker of Acute Inflammation in Humans. Frontiers in immunology, 12.

Senjor, E., Perišić Nanut, M., Breznik, B., Mitrović, A., Mlakar, J., Rotter, A., ... & Kos, J. (2021). Cystatin F acts as a mediator of immune suppression in glioblastoma. Cellular Oncology, 44(5), 1051-1063.

Yang, C., Yu, T., Liu, Z., Ye, X., Liao, X., Wang, X., ... & Peng, T. (2019). Cystatin F as a key family 2 cystatin subunit and prognostic biomarker for early‑stage pancreatic ductal adenocarcinoma. Oncology reports, 42(1), 79-90.

Dautović, E., Nanut, M. P., Softić, A., & Kos, J. (2018). The transcription factor C/EBP α controls the role of cystatin F during the differentiation of monocytes to macrophages. European Journal of Cell Biology, 97(7), 463-473.

Kos, J., Nanut, M. P., Prunk, M., Sabotič, J., Dautović, E., & Jewett, A. (2018). Cystatin F as a regulator of immune cell cytotoxicity. Cancer Immunology, Immunotherapy, 67(12), 1931-1938.

Nuvolone, M., Schmid, N., Miele, G., Sorce, S., Moos, R., Schori, C., ... & Aguzzi, A. (2017). Cystatin F is a biomarker of prion pathogenesis in mice. PLoS One, 12(2), e0171923.

Perišić Nanut, M., Sabotič, J., Švajger, U., Jewett, A., & Kos, J. (2017). Cystatin F affects natural killer cell cytotoxicity. Frontiers in immunology, 8, 1459.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CST7 protein (CAT#: V2LY-0526-LY3478)

Human Recombinant CST7 protein, hFc Tag (CAT#: V2LY-0526-LY3479)

Human Recombinant CST7 protein, His Tag (CAT#: V2LY-0526-LY3480)

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Human Recombinant CST7 protein (CAT#: V2LY-0526-LY3478)

Mouse Anti-CST7 Recombinant Antibody (CBCNC-431) (CAT#: V2LY-0125-LY152)

Mouse Anti-CST7 Recombinant Antibody (C5040) (CAT#: V2LY-0125-LY149)

Mouse Anti-CST7 Recombinant Antibody (C4549) (CAT#: V2LY-0125-LY148)

Human Recombinant CST7 protein, hFc Tag (CAT#: V2LY-0526-LY3479)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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