Human DDIT4 ELISA Kit (V2LY-0626-LY4499)

Name: Human DDIT4 ELISA Kit
SKU: V2LY-0626-LY4499
Rating: 5 (1 reviews)

See all DDIT4 antibodies

Compare Online Inquiry

Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.018 ng/mL

Detection Range

0.05-20 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

25.4 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

DNA Damage Inducible Transcript 4

Function

Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity).

Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity).

Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.

Biological Process

Apoptotic process Source: GO_Central
Brain development Source: UniProtKB
Cellular response to dexamethasone stimulus Source: Ensembl
Defense response to virus Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
Negative regulation of glycolytic process Source: Ensembl
Negative regulation of intracellular signal transduction Source: UniProtKB
Negative regulation of peptidyl-serine phosphorylation Source: UniProtKB
Negative regulation of peptidyl-threonine phosphorylation Source: UniProtKB
Negative regulation of TOR signaling Source: UniProtKB
Neuron differentiation Source: UniProtKB
Neuron migration Source: UniProtKB
Neurotrophin TRK receptor signaling pathway Source: UniProtKB
Positive regulation of neuron death Source: UniProtKB
Protein-containing complex disassembly Source: Ensembl
Reactive oxygen species metabolic process Source: Ensembl
Regulation of TOR signaling Source: GO_Central
Response to hypoxia Source: UniProtKB

Cellular Location

Cytosol; Mitochondrion

PTM

Phosphorylated by GSK3B; this promotes proteasomal degradation.
Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation.

Fattahi, F., Saeednejad Zanjani, L., Habibi Shams, Z., Kiani, J., Mehrazma, M., Najafi, M., & Madjd, Z. (2021). High expression of DNA damage-inducible transcript 4 (DDIT4) is associated with advanced pathological features in the patients with colorectal cancer. Scientific Reports, 11(1), 1-17.

Miao, Z. F., Sun, J. X., Adkins-Threats, M., Pang, M. J., Zhao, J. H., Wang, X., ... & Mills, J. C. (2021). DDIT4 licenses only healthy cells to proliferate during injury-induced metaplasia. Gastroenterology, 160(1), 260-271.

Ho, K. H., Chen, P. H., Chou, C. M., Shih, C. M., Lee, Y. T., Cheng, C. H., & Chen, K. C. (2020). A key role of DNA damage-inducible transcript 4 (DDIT4) connects autophagy and GLUT3-mediated stemness to desensitize temozolomide efficacy in glioblastomas. Neurotherapeutics, 17(3), 1212-1227.

Zhang, X., Luo, F., Li, J., Wan, J., Zhang, L., Li, H., ... & Zhao, H. (2019). DNA damage‐inducible transcript 4 is an innate guardian for human squamous cell carcinoma and an molecular vector for anti‐carcinoma effect of 1, 25 (OH) 2D3. Experimental dermatology, 28(1), 45-52.

Foltyn, M., Luger, A. L., Lorenz, N. I., Sauer, B., Mittelbronn, M., Harter, P. N., ... & Ronellenfitsch, M. W. (2019). The physiological mTOR complex 1 inhibitor DDIT4 mediates therapy resistance in glioblastoma. British journal of cancer, 120(5), 481-487.

Tirado-Hurtado, I., Fajardo, W., & Pinto, J. A. (2018). DNA damage inducible transcript 4 gene: the switch of the metabolism as potential target in cancer. Frontiers in oncology, 8, 106.

Yang, J., Zhang, Y., Tong, J., Lv, H., Zhang, C., & Chen, Z. J. (2018). Dysfunction of DNA damage-inducible transcript 4 in the decidua is relevant to the pathogenesis of preeclampsia. Biology of Reproduction, 98(6), 821-833.

Du, F., Sun, L., Chu, Y., Li, T., Lei, C., Wang, X., ... & Fan, D. (2018). DDIT4 promotes gastric cancer proliferation and tumorigenesis through the p53 and MAPK pathways. Cancer Communications, 38(1), 1-14.

Zhang, F., Liu, G., Li, D., Wei, C., & Hao, J. (2018). DDIT4 and associated lncDDIT4 modulate Th17 differentiation through the DDIT4/TSC/mTOR pathway. The Journal of Immunology, 200(5), 1618-1626.

Pinto, J. A., Rolfo, C., Raez, L. E., Prado, A., Araujo, J. M., Bravo, L., ... & Gomez, H. L. (2017). In silico evaluation of DNA Damage Inducible Transcript 4 gene (DDIT4) as prognostic biomarker in several malignancies. Scientific reports, 7(1), 1-11.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human DDIT4 ELISA Kit?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

DDIT4 Matched Antibody Pair (331) (CAT#: APMAB-331LY)

Antibody Pairs

DDIT4 Matched Antibody Pair (331) (CAT#: APMAB-331LY)

Related Products

DDIT4 Matched Antibody Pair (331) (CAT#: APMAB-331LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry