Human GPLD1 ELISA Kit (V2LY-0626-LY5919)

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Datasheet
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Basic Information

Sensitivity
0.53 ng/mL
Detection Range
1-400 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
92.3 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Glycosylphosphatidylinositol Specific Phospholipase D1
Function
This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
Biological Process
Cell migration involved in sprouting angiogenesis Source: UniProtKB
Cellular response to calcium ion Source: UniProtKB
Cellular response to cholesterol Source: UniProtKB
Cellular response to drug Source: UniProtKB
Cellular response to insulin stimulus Source: UniProtKB
Cellular response to pH Source: UniProtKB
Cellular response to triglyceride Source: UniProtKB
Chondrocyte differentiation Source: UniProtKB
Complement receptor mediated signaling pathway Source: UniProtKB
C-terminal protein lipidation Source: Reactome
GPI anchor release Source: UniProtKB
Hematopoietic stem cell migration Source: UniProtKB
Hematopoietic stem cell migration to bone marrow Source: UniProtKB
Insulin receptor signaling pathway Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of triglyceride catabolic process Source: UniProtKB
Ossification Source: UniProtKB
Phosphatidylcholine metabolic process Source: UniProtKB
Positive regulation of alkaline phosphatase activity Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of endothelial cell migration Source: UniProtKB
Positive regulation of glucose metabolic process Source: UniProtKB
Positive regulation of high-density lipoprotein particle clearance Source: UniProtKB
Positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
Positive regulation of membrane protein ectodomain proteolysis Source: UniProtKB
Positive regulation of secretion Source: UniProtKB
Positive regulation of triglyceride biosynthetic process Source: UniProtKB
Regulation of cellular response to insulin stimulus Source: UniProtKB
Response to glucose Source: UniProtKB
Transepithelial transport Source: UniProtKB
Cellular Location
Secreted

Heidarianpour, A., Keshvari, M., Shahidi, S., & Zarei, M. (2023). Modulation of GPC-4 and GPLD1 serum levels by improving glycemic indices in type 2 diabetes: Resistance training and hawthorn extract intervention. Heliyon, 9(4).

Li, X., Shi, X., McPherson, M., Hager, M., Garcia, G. G., & Miller, R. A. (2022). Cap‐independent translation of GPLD1 enhances markers of brain health in long‐lived mutant and drug‐treated mice. Aging Cell, 21(9), e13685.

Kruse, A., Bischof, E., Raaz-Schrauder, D., Achenbach, S., Dietel, B., Tauchi, M., & Urschel, K. (2022). Decreasing GPLD 1-levels in patients after ST-elevated myocardial infarction. Atherosclerosis, 355, 121.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Gpld1 ELISA Kit (CAT#: V2LY-0626-LY1657)

Related Products

Mouse Anti-GPLD1 Recombinant Antibody (38A1) (CAT#: CBMAB-G4600-LY)

Mouse Anti-GPLD1 Recombinant Antibody (CBLG1-1621) (CAT#: V2LY-0226-LY771)

Rat Gpld1 ELISA Kit (CAT#: V2LY-0626-LY1657)

Mouse Anti-GPLD1 Recombinant Antibody (15) (CAT#: CBMAB-G0699-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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