Human HABP2 ELISA Kit (V2LY-0626-LY3912)

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Tested Data
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Basic Information

Sensitivity
0.056 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
62.7 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
hyaluronan binding protein 2
Function
Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII (PubMed:8827452, PubMed:10754382, PubMed:11217080).

May function as a tumor suppressor negatively regulating cell proliferation and cell migration (PubMed:26222560).
Biological Process
Cell adhesion Source: ProtInc
Proteolysis Source: MGI
Cellular Location
Secreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
Involvement in disease
Thyroid cancer, non-medullary, 5 (NMTC5):
A form of non-medullary thyroid cancer (NMTC), a cancer characterized by tumors originating from the thyroid follicular cells. NMTCs represent approximately 95% of all cases of thyroid cancer and are classified into papillary, follicular, Hurthle cell, and anaplastic neoplasms.
PTM
Proteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-313 or Lys-319 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Lys-169 or Arg-170 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-480 to give rise to inactive 17 kDa and 8 kDa fragments (By similarity).

Luo, H., Huang, Q., Huang, D., Liu, C., Long, X., & Tang, R. (2023). HABP2 Encapsulated by Peripheral Blood-Derived Exosomes Suppresses Astrocyte Autophagy to Exacerbate Neuroinflammatory Injury in Mice with Ischemic Stroke. ACS Chemical Neuroscience.

Cheng, L., Wang, D., Wang, Z., Li, H., Wang, G., Wu, Z., ... & Li, Y. (2022). Proteomic landscape mapping of organ-resolved Behçet's syndrome using in-depth plasma proteomics for identifying HABP2 expression associated with vascular involvement. Arthritis & Rheumatology (Hoboken, NJ).

Jiang, Y., Li, J., Sang, C., Cao, G., & Wang, S. (2020). Diagnostic and prognostic value of HABP2 as a novel biomarker for endometrial cancer. Annals of Translational Medicine, 8(18).

de Randamie, R., Martos-Moreno, G. Á., Lumbreras, C., Chueca, M., Donnay, S., Luque, M., ... & Moreno, J. C. (2018). Frequent and rare HABP2 variants are not associated with increased Susceptibility to familial nonmedullary Thyroid carcinoma in the Spanish population. Hormone Research in Paediatrics, 89(6), 397-407.

Husseini-Akram, F., Haroun, S., Altmäe, S., Skjöldebrand-Sparre, L., Åkerud, H., Poromaa, I. S., ... & Stavreus-Evers, A. (2018). Hyaluronan–binding protein 2 (HABP2) gene variation in women with recurrent miscarriage. BMC women's health, 18, 1-6.

Colombo, C., Fugazzola, L., Muzza, M., Proverbio, M. C., & Cirello, V. (2018). Letter regarding the article:“Multiple HABP2 variants in familial papillary thyroid carcinoma: Contribution of a group of “thyroid-checked” controls” by Kern et al. European Journal of Medical Genetics, 61(2), 104-105.

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For research use only. Not intended for any clinical use.

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