Human HDGF ELISA Kit (V2LY-0626-LY3913)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.031 ng/mL
Detection Range
0.05-30 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
26.8 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Heparin Binding Growth Factor
Function
Isoform 1:
Acts as a transcriptional repressor (PubMed:17974029).

Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719).

Heparin-binding protein (PubMed:15491618).

Isoform 2:
Does not have mitogenic activity for fibroblasts (PubMed:26845719).

Does not bind heparin (PubMed:26845719).

Isoform 3:
Has mitogenic activity for fibroblasts (PubMed:26845719).

Heparin-binding protein (PubMed:26845719).
Biological Process
Cellular response to interleukin-7 Source: Ensembl
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of cell division Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Protein localization to nucleus Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction Source: ProtInc
Cellular Location
Isoform 1: Nucleus; Extracellular exosome; Cytoplasm. Secreted by exosomes and is located inside the exosome (PubMed:27926477). May also be secreted as free protein via an as yet unknown pathway (PubMed:27926477).
Isoform 2: Nucleus; Extracellular exosome; Cytoplasm. Secreted by exosomes and is located on the outer exosome surface.
Isoform 3: Nucleus; Extracellular exosome; Cytoplasm. Secreted by exosomes and is located on the outer exosome surface.
PTM
Sumoylated with SUMO1. Sumoylation prevents binding to chromatin.
Phosphorylation at Ser-165 is likely to be required for secretion.

Fang, Y., & Yang, Q. (2022). Specificity protein 1-induced serine peptidase inhibitor, Kunitz type 1 antisense RNA1 regulates colorectal cancer cell proliferation, migration, invasion and apoptosis through targeting heparin binding growth factor via sponging microRNA-214. Bioengineered, 13(2), 3309-3322.

Qiu, L., Ma, Y., Chen, X., Zhou, L., Zhang, H., Zhong, G., ... & Tang, J. (2021). Heparin-binding growth factor (HDGF) drives radioresistance in breast cancer by activating the STAT3 signaling pathway. Journal of Translational Medicine, 19(1), 344.

Xia, C., Li, Q., Cheng, X., Wu, T., & Gao, P. (2021). miR-4323 targets hepatoma-derived growth factor (HDGF) to suppress colorectal cancer cell proliferation. Pathology-Research and Practice, 225, 153544.

Keppie, S. J., Mansfield, J. C., Tang, X., Philp, C. J., Graham, H. K., Önnerfjord, P., ... & Vincent, T. L. (2021). Matrix-bound growth factors are released upon cartilage compression by an aggrecan-dependent sodium flux that is lost in osteoarthritis. Function, 2(5), zqab037.

Keppie, S. J. (2021). Bioavailability of matrix-bound growth factors in cartilage injury (Doctoral dissertation, University of Oxford).

Eguchi, R., & Wakabayashi, I. (2020). HDGF enhances VEGF‑dependent angiogenesis and FGF‑2 is a VEGF‑independent angiogenic factor in non‑small cell lung cancer. Oncology reports, 44(1), 14-28.

Zhang, C., Chang, X., Chen, D., Yang, F., Li, Z., Li, D., ... & Xu, Z. (2019). Downregulation of HDGF inhibits the tumorigenesis of bladder cancer cells by inactivating the PI3K-AKT signaling pathway. Cancer Management and Research, 7909-7923.

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For research use only. Not intended for any clinical use.

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