Human ITIH4 ELISA Kit (V2LY-0626-LY3416)

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Tested Data
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Basic Information

Sensitivity
0.13 ng/mL
Detection Range
0.3-90 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
103.4 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Inter-Alpha-Trypsin Inhibitor Heavy Chain Family Member 4
Function
Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
Biological Process
Acute-phase responseManual Assertion Based On ExperimentIEP:UniProtKB
Hyaluronan metabolic processIEA:InterPro
Response to cytokineManual Assertion Based On ExperimentIEP:UniProtKB
Cellular Location
Secreted
PTM
Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
N- and O-glycosylated. In urine, O-linked glycosylation on threonine residues in the region from Thr-719 to Thr-725 consists of core 1 or possibly core 8 glycans. Mainly Hex(HexNAc)(2), but also some Hex(3)(HexNAc)(3). N-glycosylated but not O-glycosylated in plasma.

Laursen, T. L., Bossen, L., Pihl, R., Troldborg, A., Sandahl, T. D., Hansen, A. G., ... & Thiel, S. (2022). Highly increased levels of inter-α-inhibitor heavy chain 4 (ITIH4) in autoimmune cholestatic liver diseases. Journal of Clinical and Translational Hepatology, 10(5), 796.

Pihl, R., Jensen, R. K., Poulsen, E. C., Jensen, L., Hansen, A. G., Thøgersen, I. B., ... & Thiel, S. (2021). ITIH4 acts as a protease inhibitor by a novel inhibitory mechanism. Science advances, 7(2), eaba7381.

Ma, Y., Li, R., Wang, J., Jiang, W., Yuan, X., Cui, J., & Wang, C. (2021). ITIH4, as an inflammation biomarker, mainly increases in bacterial bloodstream infection. Cytokine, 138, 155377.

Sun, Y., Jin, J., Jing, H., Lu, Y., Zhu, Q., Shu, C., ... & Jing, D. (2021). ITIH4 is a novel serum biomarker for early gastric cancer diagnosis. Clinica Chimica Acta, 523, 365-373.

Soler, L., Dąbrowski, R., García, N., Alava, M. A., Lampreave, F., Piñeiro, M., ... & Bochniarz, M. (2019). Acute-phase inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) levels in serum and milk of cows with subclinical mastitis caused by Streptococcus species and coagulase-negative Staphylococcus species. Journal of dairy science, 102(1), 539-546.

Shi, X., Ohta, Y., Liu, X., Shang, J., Morihara, R., Nakano, Y., ... & Abe, K. (2019). Acute anti-inflammatory markers ITIH4 and AHSG in mice brain of a novel Alzheimer’s disease model. Journal of Alzheimer's Disease, 68(4), 1667-1675.

Liang, C., Zhao, T., Li, H., He, F., Zhao, X., Zhang, Y., ... & Guo, J. (2019). Long non-coding RNA ITIH4-AS1 accelerates the proliferation and metastasis of colorectal cancer by activating JAK/STAT3 signaling. Molecular Therapy-Nucleic Acids, 18, 183-193.

Nakamura, N., Hatano, E., Iguchi, K., Sato, M., Kawaguchi, H., Ohtsu, I., ... & Ikegawa, M. (2019). Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study. BMC cancer, 19(1), 1-14.

Li, X., Li, B., Li, B., Guo, T., Sun, Z., Li, X., ... & Zeng, Y. (2018). ITIH4: effective serum marker, early warning and diagnosis, hepatocellular carcinoma. Pathology & Oncology Research, 24, 663-670.

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For research use only. Not intended for any clinical use.

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