Human Recombinant ITIH4 protein, His Tag (V2LY-0526-LY5081)

Name: Human Recombinant ITIH4 protein, His Tag
SKU: V2LY-0526-LY5081
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ITIH4 protein, His Tag consist of Amino Acid: 1-930 and predicts a molecular mass of 101.76 kDa.

Molecule Mass

101.76 kDa

Verified

HPLC

Sequence

Amino Acid: 1-930

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile NaH2PO4, EDTA, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Inter-Alpha-Trypsin Inhibitor Heavy Chain Family Member 4

Function

Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.

Biological Process

Acute-phase responseManual Assertion Based On ExperimentIEP:UniProtKB
Hyaluronan metabolic processIEA:InterPro
Response to cytokineManual Assertion Based On ExperimentIEP:UniProtKB

Cellular Location

Secreted

PTM

Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
N- and O-glycosylated. In urine, O-linked glycosylation on threonine residues in the region from Thr-719 to Thr-725 consists of core 1 or possibly core 8 glycans. Mainly Hex(HexNAc)(2), but also some Hex(3)(HexNAc)(3). N-glycosylated but not O-glycosylated in plasma.

Laursen, T. L., Bossen, L., Pihl, R., Troldborg, A., Sandahl, T. D., Hansen, A. G., ... & Thiel, S. (2022). Highly increased levels of inter-α-inhibitor heavy chain 4 (ITIH4) in autoimmune cholestatic liver diseases. Journal of Clinical and Translational Hepatology, 10(5), 796.

Pihl, R., Jensen, R. K., Poulsen, E. C., Jensen, L., Hansen, A. G., Thøgersen, I. B., ... & Thiel, S. (2021). ITIH4 acts as a protease inhibitor by a novel inhibitory mechanism. Science advances, 7(2), eaba7381.

Ma, Y., Li, R., Wang, J., Jiang, W., Yuan, X., Cui, J., & Wang, C. (2021). ITIH4, as an inflammation biomarker, mainly increases in bacterial bloodstream infection. Cytokine, 138, 155377.

Sun, Y., Jin, J., Jing, H., Lu, Y., Zhu, Q., Shu, C., ... & Jing, D. (2021). ITIH4 is a novel serum biomarker for early gastric cancer diagnosis. Clinica Chimica Acta, 523, 365-373.

Soler, L., Dąbrowski, R., García, N., Alava, M. A., Lampreave, F., Piñeiro, M., ... & Bochniarz, M. (2019). Acute-phase inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) levels in serum and milk of cows with subclinical mastitis caused by Streptococcus species and coagulase-negative Staphylococcus species. Journal of dairy science, 102(1), 539-546.

Shi, X., Ohta, Y., Liu, X., Shang, J., Morihara, R., Nakano, Y., ... & Abe, K. (2019). Acute anti-inflammatory markers ITIH4 and AHSG in mice brain of a novel Alzheimer’s disease model. Journal of Alzheimer's Disease, 68(4), 1667-1675.

Liang, C., Zhao, T., Li, H., He, F., Zhao, X., Zhang, Y., ... & Guo, J. (2019). Long non-coding RNA ITIH4-AS1 accelerates the proliferation and metastasis of colorectal cancer by activating JAK/STAT3 signaling. Molecular Therapy-Nucleic Acids, 18, 183-193.

Nakamura, N., Hatano, E., Iguchi, K., Sato, M., Kawaguchi, H., Ohtsu, I., ... & Ikegawa, M. (2019). Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study. BMC cancer, 19(1), 1-14.

Li, X., Li, B., Li, B., Guo, T., Sun, Z., Li, X., ... & Zeng, Y. (2018). ITIH4: effective serum marker, early warning and diagnosis, hepatocellular carcinoma. Pathology & Oncology Research, 24, 663-670.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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