Human KLF15 ELISA Kit (V2LY-0626-LY4223)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.11 ng/mL
Detection Range
0.2-60 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
44.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Kruppel Like Factor 15
Function
Lysosomal transporter which is essential for liver homeostasis. Required to maintain stability and lysosomal localization of GLMP.
Biological Process
Protein localization to lysosomeISS:UniProtKB
Protein stabilizationISS:UniProtKB
Cellular Location
Lysosome membrane
PTM
Not N-glycosylated.

Fan, L., Sweet, D. R., Fan, E. K., Prosdocimo, D. A., Madera, A., Jiang, Z., ... & Jain, M. K. (2022). Transcription factors KLF15 and PPARδ cooperatively orchestrate genome-wide regulation of lipid metabolism in skeletal muscle. Journal of Biological Chemistry, 298(6).

Suzuki, N., Kanai, A., Suzuki, Y., Ogino, H., & Ochi, H. (2022). Adrenergic receptor signaling induced by Klf15, a regulator of regeneration enhancer, promotes kidney reconstruction. Proceedings of the National Academy of Sciences, 119(33), e2204338119.

Anzai, K., Tsuruya, K., Ida, K., Kagawa, T., Inagaki, Y., & Kamiya, A. (2021). Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts. Scientific Reports, 11(1), 18551.

Zhao, Y., Song, W., Wang, L., Rane, M. J., Han, F., & Cai, L. (2019). Multiple roles of KLF15 in the heart: Underlying mechanisms and therapeutic implications. Journal of Molecular and Cellular Cardiology, 129, 193-196.

Li, Y., Zhao, M., & Xiao, W. (2018). KLF15 regulates the expression of MMP-3 in human chondrocytes. Journal of Interferon & Cytokine Research, 38(8), 356-362.

Guo, H., Khan, R., Raza, S. H. A., Ning, Y., Wei, D., Wu, S., ... & Zan, L. (2018). KLF15 promotes transcription of KLF3 gene in bovine adipocytes. Gene, 659, 77-83.

Wang, X., He, M., Li, J., Wang, H., & Huang, J. (2018). KLF15 suppresses cell growth and predicts prognosis in lung adenocarcinoma. Biomedicine & Pharmacotherapy, 106, 672-677.

Gu, X., Xu, D., Fu, L., Wang, Y., Mei, C., & Gao, X. (2018). KLF 15 works as an early anti-fibrotic transcriptional regulator in Ang II-induced renal fibrosis via down-regulation of CTGF expression. Kidney and Blood Pressure Research, 42(6), 999-1012.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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