Human LDHB ELISA Kit (V2LY-0626-LY4821)

Name: Human LDHB ELISA Kit
SKU: V2LY-0626-LY4821
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

7.22 mU/mL

Detection Range

15-3000 mU/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

36.6 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

LDHB

Biological Process

Carbohydrate metabolic processIEA:InterPro
Lactate metabolic processManual Assertion Based On ExperimentIDA:ComplexPortal
Pyruvate metabolic processManual Assertion Based On ExperimentIDA:ComplexPortal

Cellular Location

Cytoplasm
Mitochondrion inner membrane

Involvement in disease

Lactate dehydrogenase B deficiency (LDHBD):
A condition with no deleterious effects on health. LDHBD is of interest to laboratory medicine mainly because it can cause misdiagnosis in those disorders in which elevation of serum LDH is expected.

Wulandari, F., & Hanifa, M. (2023). Integrative bioinformatics reveals the lactate dehydrogenase B (LDHB) significance in colon adenocarcinoma. Molecular and Cellular Biomedical Sciences, 7(2), 98-108.

Guo, B., Shu, H., Luo, L., Liu, X., Ma, Y., Zhang, J., ... & Zhang, C. (2023). Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in Mice. Nutrients, 15(22), 4846.

Deng, H., Gao, Y., Trappetti, V., Hertig, D., Karatkevich, D., Losmanova, T., ... & Marti, T. M. (2022). Targeting lactate dehydrogenase B-dependent mitochondrial metabolism affects tumor initiating cells and inhibits tumorigenesis of non-small cell lung cancer by inducing mtDNA damage. Cellular and molecular life sciences, 79(8), 445.

Wang, R., Li, J., Zhang, C., Guan, X., Qin, B., Jin, R., ... & Cheng, L. (2022). Lactate dehydrogenase B is required for pancreatic cancer cell immortalization through activation of telomerase activity. Frontiers in Oncology, 12, 821620.

Shibata, S., Sogabe, S., Miwa, M., Fujimoto, T., Takakura, N., Naotsuka, A., ... & Soga, T. (2021). Identification of the first highly selective inhibitor of human lactate dehydrogenase B. Scientific reports, 11(1), 21353.

Wang, T., Chen, K., Yao, W., Zheng, R., He, Q., Xia, J., ... & Huang, F. (2021). Acetylation of lactate dehydrogenase B drives NAFLD progression by impairing lactate clearance. Journal of Hepatology, 74(5), 1038-1052.

Frank, A. C., Raue, R., Fuhrmann, D. C., Sirait-Fischer, E., Reuse, C., Weigert, A., ... & Brüne, B. (2021). Lactate dehydrogenase B regulates macrophage metabolism in the tumor microenvironment. Theranostics, 11(15), 7570.

Papaneophytou, C., Zervou, M. E., & Theofanous, A. (2021). Optimization of a colorimetric assay to determine lactate dehydrogenase B activity using design of experiments. SLAS DISCOVERY: Advancing the Science of Drug Discovery, 26(3), 383-399.

Hong, S. M., Lee, Y. K., Park, I., Kwon, S. M., Min, S., & Yoon, G. (2019). Lactic acidosis caused by repressed lactate dehydrogenase subunit B expression down-regulates mitochondrial oxidative phosphorylation via the pyruvate dehydrogenase (PDH)–PDH kinase axis. Journal of Biological Chemistry, 294(19), 7810-7820.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Ldhb ELISA Kit (CAT#: V2LY-0626-LY2540)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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