Human LPO ELISA Kit (V2LY-0626-LY4160)

Name: Human LPO ELISA Kit
SKU: V2LY-0626-LY4160
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.023 ng/mL

Detection Range

0.05-9 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

80.3 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

LPO

Function

Antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN) (By similarity).
May contribute to airway host defense against infection.

Biological Process

Defense response to bacteriumManual Assertion Based On ExperimentIDA:UniProtKB
Detection of chemical stimulus involved in sensory perception of bitter tasteManual Assertion Based On ExperimentIDA:UniProtKB
Hydrogen peroxide catabolic processIEA:UniProtKB-KW
Response to oxidative stress1 PublicationNAS:UniProtKB

Cellular Location

Secreted

Yamakaze, J., & Lu, Z. (2021). Deletion of the lactoperoxidase gene causes multisystem inflammation and tumors in mice. Scientific Reports, 11(1), 12429.

Welk, A., Patjek, S., Gärtner, M., Baguhl, R., Schwahn, C., & Below, H. (2021). Antibacterial and antiplaque efficacy of a lactoperoxidase-thiocyanate-hydrogen-peroxide-system-containing lozenge. BMC microbiology, 21, 1-12.

Viswanathan, V., Rani, C., Ahmad, N., Singh, P. K., Sharma, P., Kaur, P., ... & Singh, T. P. (2021). Structure of yak lactoperoxidase at 1.55 Å resolution. The Protein Journal, 40, 8-18.

Buys, E. M., & Seifu, E. (2021). Enzymes indigenous to milk: Lactoperoxidase. Elsevier.

El-Fakharany, E. M. (2021). Nanoformulation approach for improved stability and efficiency of lactoperoxidase. Preparative Biochemistry & Biotechnology, 51(7), 629-641.

Al-Shehri, S. S., Duley, J. A., & Bansal, N. (2020). Xanthine oxidase-lactoperoxidase system and innate immunity: Biochemical actions and physiological roles. Redox biology, 34, 101524.

Kalin, R., Koksal, Z., Kalin, P., Karaman, M., Gulcin, İ., & Ozdemir, H. (2020). In vitro effects of standard antioxidants on lactoperoxidase enzyme–A molecular docking approach. Journal of Biochemical and Molecular Toxicology, 34(1), e22421.

Köksal, Z., & Alim, Z. (2020). Lactoperoxidase, an antimicrobial enzyme, is inhibited by some indazoles. Drug and chemical toxicology, 43(1), 22-26.

Magacz, M., Kędziora, K., Sapa, J., & Krzyściak, W. (2019). The significance of lactoperoxidase system in oral health: Application and efficacy in oral hygiene products. International journal of molecular sciences, 20(6), 1443.

Al-Baarri, A. N. M., Damayanti, N. T., Legowo, A. M., Tekiner, İ. H., & Hayakawa, S. (2019). Enhanced antibacterial activity of lactoperoxidase–thiocyanate–hydrogen peroxide system in reduced-lactose milk whey. International Journal of Food Science, 2019.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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