Human MAGEA1 ELISA Kit (V2LY-0626-LY1870)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0092 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
34.3 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
MAGE Family Member A1
Function
May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
Biological Process
Negative regulation of Notch signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Nucleus

Mastutik, G., Rahniayu, A., Marhana, I. A., Kurniasari, N., Rahaju, A. S., Amin, M., & Trianto, H. F. (2023). The MAGE A1-A10 Expression associated with Histopathological Findings of Malignant or Non-Malignant Cells in Peripheral Lung Tumors. Asian Pacific Journal of Cancer Prevention: APJCP, 24(7), 2329.

Bassan, D., Gozlan, Y. M., Sharbi‐Yunger, A., Tzehoval, E., Greenstein, E., Bitan, L., ... & Eisenbach, L. (2021). Avidity optimization of a MAGE‐A1‐specific TCR with somatic hypermutation. European journal of immunology, 51(6), 1505-1518.

Jeong, Y. J., Park, S. H., & Jeon, C. H. (2020). Detection of circulating tumor cells in patients with breast cancer using the conditionally reprogrammed cell culture method and reverse transcription‑PCR of hTERT and MAGE A1‑6. Oncology Letters, 20(4), 1-1.

Liu, S., Zhao, Y., Xu, Y., Sang, M., Zhao, R., Gu, L., & Shan, B. (2020). The clinical significance of methylation of MAGE-A1 and-A3 promoters and expression of DNA methyltransferase in patients with laryngeal squamous cell carcinoma. American Journal of Otolaryngology, 41(1), 102318.

Mi, Y., Liu, F., Liang, X., Liu, S., Huang, X., Sang, M., & Geng, C. (2019). Tumor suppressor let-7a inhibits breast cancer cell proliferation, migration and invasion by targeting MAGE-A1. Neoplasma, 66(1), 54-62.

Mao, Y., Fan, W., Hu, H., Zhang, L., Michel, J., Wu, Y., ... & Tang, Q. (2019). MAGE-A1 in lung adenocarcinoma as a promising target of chimeric antigen receptor T cells. Journal of hematology & oncology, 12, 1-18.

Srdelic, S., Kuzmic-Prusac, I., Spagnoli, G. C., Juretic, A., & Capkun, V. (2019). MAGE-A4 and MAGE-A1 immunohistochemical expression in high-grade endometrial cancer. International Journal of Gynecological Pathology, 38(1), 59-65.

Zhao, J., Wang, Y., Liang, Q., Xu, Y., & Sang, J. (2019). MAGEA1 inhibits the expression of BORIS via increased promoter methylation. Journal of Cell Science, 132(1), jcs218628.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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