Human MCHR1 ELISA Kit (V2LY-0626-LY4226)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.23 ng/mL
Detection Range
0.5-60 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
46.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
MELANIN CONCENTRATING HORMONE RECEPTOR 1
Function
Receptor for melanin-concentrating hormone, coupled to both G proteins that inhibit adenylyl cyclase and G proteins that activate phosphoinositide hydrolysis.
Biological Process
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: ProtInc
Cell surface receptor signaling pathway Source: Ensembl
Feeding behavior Source: ProtInc
Generation of precursor metabolites and energy Source: ProtInc
G protein-coupled receptor signaling pathway Source: ProtInc
Neuropeptide signaling pathway Source: GO_Central
Positive regulation of calcium ion transport Source: Ensembl
Positive regulation of cytosolic calcium ion concentration Source: ProtInc
Cellular Location
Cell membrane
Topology
Extracellular: 1-113
Helical: 114-136
Cytoplasmic: 137-148
Helical: 149-171
Extracellular: 172-185
Helical: 186-208
Cytoplasmic: 209-227
Helical: 228-250
Extracellular: 251-278
Helical: 279-301
Cytoplasmic: 302-321
Helical: 322-344
Extracellular: 345-358
Helical: 359-381
Cytoplasmic: 382-422

Sariyer, E., & Sariyer, A. S. (2023). Computational prediction of analog compounds of the membrane protein MCHR1 antagonists ALB-127158 and KRX-104130. Journal of Bioenergetics and Biomembranes, 1-12.

Huszár, J., Bozó, É., Beke, G., Katalin Szalai, K., Kardos, P., Boros, A., ... & Éles, J. (2022). hERG Optimization of Benzofuro− Pyridine and Pyrazino− Indole Derivatives as MCHR1 Antagonists. ChemMedChem, 17(7), e202100707.

Lim, G., You, K. Y., Lee, J. H., Jeon, M. K., Lee, B. H., Ryu, J. Y., & Oh, K. S. (2022). Identification and new indication of melanin-concentrating hormone receptor 1 (MCHR1) antagonist derived from machine learning and transcriptome-based drug repositioning approaches. International Journal of Molecular Sciences, 23(7), 3807.

Wang, M., Zhai, Y., Lei, X., Xu, J., Jiang, B., Kuang, Z., ... & Zhang, C. (2022). Determination of the Interaction and Pharmacological Modulation of MCHR1 Signaling by the C-Terminus of MRAP2 Protein. Frontiers in Endocrinology, 13, 848728.

Takamura, N., Renaud, L., da Silveira, W. A., & Feghali-Bostwick, C. (2021). PDGF promotes dermal fibroblast activation via a novel mechanism mediated by signaling through MCHR1. Frontiers in Immunology, 12, 745308.

Hsiao, Y. C., Muñoz-Estrada, J., Tuz, K., & Ferland, R. J. (2021). The transition zone protein AHI1 regulates neuronal ciliary trafficking of MCHR1 and its downstream signaling pathway. Journal of Neuroscience, 41(17), 3932-3943.

Diniz, G. B., Battagello, D. S., Klein, M. O., Bono, B. S., Ferreira, J. G., Motta‐Teixeira, L. C., ... & Bittencourt, J. C. (2020). Ciliary melanin‐concentrating hormone receptor 1 (MCHR1) is widely distributed in the murine CNS in a sex‐independent manner. Journal of neuroscience research, 98(10), 2045-2071.

Pałasz, A., Piwowarczyk-Nowak, A., Suszka-Świtek, A., Bogus, K., Filipczyk, Ł., Vecchia, A. D., ... & Wiaderkiewicz, R. (2020). Escitalopram as a modulator of proopiomelanocortin, kisspeptin, Kiss1R and MCHR1 gene expressions in the male rat brain. Molecular Biology Reports, 47, 8273-8278.

Balber, T., Benčurová, K., Kiefer, F. W., Kulterer, O. C., Klebermass, E. M., Egger, G., ... & Philippe, C. (2019). In vitro radiopharmaceutical evidence for MCHR1 binding sites in murine brown adipocytes. Frontiers in Endocrinology, 10, 324.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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