Human ME1 ELISA Kit (V2LY-0626-LY4021)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.107 ng/mL
Detection Range
0.2-60 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
64.2 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
malic enzyme 1, NADP(+)-dependent, cytosolic
Function
Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP+ and divalent metal ions, and decarboxylation of oxaloacetate.
Biological Process
Carbohydrate metabolic process Source: ProtInc
Malate metabolic process Source: UniProtKB
Nucleotide biosynthetic process Source: UniProtKB
Pyruvate metabolic process Source: GO_Central
Regulation of NADP metabolic process Source: CACAO
Response to carbohydrate Source: UniProtKB
Response to hormone Source: UniProtKB
Cellular Location
Cytoplasm

Simmen, F. A., Pabona, J. M. P., Al-Dwairi, A., Alhallak, I., Montales, M. T. E., & Simmen, R. C. (2023). Malic Enzyme 1 (ME1) Promotes Adiposity and Hepatic Steatosis and Induces Circulating Insulin and Leptin in Obese Female Mice. International Journal of Molecular Sciences, 24(7), 6613.

Fang, X., Zhang, J., Li, Y., Song, Y., Yu, Y., Cai, Z., ... & Wang, F. (2023). Malic Enzyme 1 as a Novel Anti‐Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury. Advanced Science, 10(13), 2205436.

Yoshida, T., Kawabe, T., Cantley, L. C., & Lyssiotis, C. A. (2022). Discovery and Characterization of a Novel Allosteric Small-Molecule Inhibitor of NADP+-Dependent Malic Enzyme 1. Biochemistry, 61(15), 1548-1553.

Badia, M. B., Maurino, V. G., Pavlovic, T., Arias, C. L., Pagani, M. A., Andreo, C. S., ... & Gerrard Wheeler, M. C. (2020). Loss of function of Arabidopsis NADP‐malic enzyme 1 results in enhanced tolerance to aluminum stress. The Plant Journal, 101(3), 653-665.

Nakashima, C., Kirita, T., Yamamoto, K., Mori, S., Luo, Y., Sasaki, T., ... & Kuniyasu, H. (2020). Malic enzyme 1 is associated with tumor budding in oral squamous cell carcinomas. International Journal of Molecular Sciences, 21(19), 7149.

Simmen, F. A., Alhallak, I., & Simmen, R. C. (2020). Malic enzyme 1 (ME1) in the biology of cancer: it is not just intermediary metabolism. Journal of molecular endocrinology, 65(4), R77-R90.

Liu, C., Cao, J., Lin, S., Zhao, Y., Zhu, M., Tao, Z., & Hu, X. (2020). Malic enzyme 1 indicates worse prognosis in breast cancer and promotes metastasis by manipulating reactive oxygen species. OncoTargets and therapy, 8735-8747.

Shi, Y., Zhou, S., Wang, P., Guo, Y., Xie, B., & Ding, S. (2019). Malic enzyme 1 (ME1) is a potential oncogene in gastric cancer cells and is associated with poor survival of gastric cancer patients. OncoTargets and therapy, 12, 5589.

Yazdanpanah, F., Maurino, V. G., Mettler-Altmann, T., Buijs, G., Bailly, M., Karimi Jashni, M., ... & Bentsink, L. (2019). NADP-MALIC ENZYME 1 affects germination after seed storage in Arabidopsis thaliana. Plant and Cell Physiology, 60(2), 318-328.

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For research use only. Not intended for any clinical use.

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