Human MMP10 ELISA Kit (2) (V2LY-0626-LY1887)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.28 ng/mL
Detection Range
0.5-150 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
54.2 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
matrix metallopeptidase 10
Function
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Biological Process
Collagen catabolic process Source: GO_Central
Extracellular matrix disassembly Source: Reactome
Extracellular matrix organization Source: GO_Central
Proteolysis Source: ProtInc
Cellular Location
Extracellular matrix

Ishimura, T., Ishii, A., Yamada, H., Osaki, K., Toda, N., Mori, K. P., ... & Yokoi, H. (2023). Matrix metalloproteinase-10 deficiency has protective effects against peritoneal inflammation and fibrosis via transcription factor NFκΒ pathway inhibition. Kidney International, 104(5), 929-942.

Abasheva, D., Dolcet-Negre, M. M., Fernández-Seara, M. A., Mora-Gutiérrez, J. M., Orbe, J., Escalada, F. J., & Garcia-Fernandez, N. (2022). Association between Circulating Levels of 25-Hydroxyvitamin D3 and Matrix Metalloproteinase-10 (MMP-10) in Patients with Type 2 Diabetes. Nutrients, 14(17), 3484.

Sun, X., & Liu, Y. (2022). Matrix metalloproteinase-10 in kidney injury repair and disease. International Journal of Molecular Sciences, 23(4), 2131.

Fonseca‐Camarillo, G., Furuzawa‐Carballeda, J., Martínez‐Benitez, B., Barreto‐Zuñiga, R., & Yamamoto‐Furusho, J. K. (2021). Increased expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and MMP10, MMP23 in inflammatory bowel disease: cross‐sectional study. Scandinavian Journal of Immunology, 93(1), e12962.

Kudelski, J., Młynarczyk, G., Gudowska-Sawczuk, M., Mroczko, B., Darewicz, B., Bruczko-Goralewska, M., ... & Romanowicz, L. (2021). Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma. Journal of Clinical Medicine, 10(16), 3683.

Zuo, Y., Wang, C., Sun, X., Hu, C., Liu, J., Hong, X., ... & Liu, Y. (2021). Identification of matrix metalloproteinase-10 as a key mediator of podocyte injury and proteinuria. Kidney International, 100(4), 837-849.

Matilla, L., Roncal, C., Ibarrola, J., Arrieta, V., García-Peña, A., Fernández-Celis, A., ... & López-Andrés, N. (2020). A role for MMP-10 (matrix metalloproteinase-10) in calcific aortic valve stenosis. Arteriosclerosis, thrombosis, and vascular biology, 40(5), 1370-1382.

Razai, A. S., Eckelman, B. P., & Salvesen, G. S. (2020). Selective inhibition of matrix metalloproteinase 10 (MMP10) with a single-domain antibody. Journal of Biological Chemistry, 295(8), 2464-2472.

Lv, Y. P., Cheng, P., Zhang, J. Y., Mao, F. Y., Teng, Y. S., Liu, Y. G., ... & Zhuang, Y. (2019). Helicobacter pylori–induced matrix metallopeptidase-10 promotes gastric bacterial colonization and gastritis. Science advances, 5(4), eaau6547.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MMP10 protein, His Tag (CAT#: V2LY-0526-LY5611)

Human MMP10 ELISA Kit (CAT#: V2LY-0626-LY1251)

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Mouse Anti-MMP10 Recombinant Antibody (CBFYM-2346) (CAT#: CBMAB-M2532-FY)

Human MMP10 ELISA Kit (CAT#: V2LY-0626-LY1251)

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Human Recombinant MMP10 protein, His Tag (CAT#: V2LY-0526-LY5611)

Isotype control

For research use only. Not intended for any clinical use.

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