Human Recombinant MMP10 protein, His Tag (V2LY-0526-LY5611)

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Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant MMP10 protein, His Tag consist of Amino Acid: 99-476 and predicts a molecular mass of 44.38 kDa.
Molecule Mass
44.38 kDa
Sequence
Amino Acid: 99-476
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
≥90% as determined by SDS-PAGE.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
matrix metallopeptidase 10
Function
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Biological Process
Collagen catabolic process Source: GO_Central
Extracellular matrix disassembly Source: Reactome
Extracellular matrix organization Source: GO_Central
Proteolysis Source: ProtInc
Cellular Location
Extracellular matrix

Ishimura, T., Ishii, A., Yamada, H., Osaki, K., Toda, N., Mori, K. P., ... & Yokoi, H. (2023). Matrix metalloproteinase-10 deficiency has protective effects against peritoneal inflammation and fibrosis via transcription factor NFκΒ pathway inhibition. Kidney International, 104(5), 929-942.

Abasheva, D., Dolcet-Negre, M. M., Fernández-Seara, M. A., Mora-Gutiérrez, J. M., Orbe, J., Escalada, F. J., & Garcia-Fernandez, N. (2022). Association between Circulating Levels of 25-Hydroxyvitamin D3 and Matrix Metalloproteinase-10 (MMP-10) in Patients with Type 2 Diabetes. Nutrients, 14(17), 3484.

Sun, X., & Liu, Y. (2022). Matrix metalloproteinase-10 in kidney injury repair and disease. International Journal of Molecular Sciences, 23(4), 2131.

Fonseca‐Camarillo, G., Furuzawa‐Carballeda, J., Martínez‐Benitez, B., Barreto‐Zuñiga, R., & Yamamoto‐Furusho, J. K. (2021). Increased expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and MMP10, MMP23 in inflammatory bowel disease: cross‐sectional study. Scandinavian Journal of Immunology, 93(1), e12962.

Kudelski, J., Młynarczyk, G., Gudowska-Sawczuk, M., Mroczko, B., Darewicz, B., Bruczko-Goralewska, M., ... & Romanowicz, L. (2021). Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma. Journal of Clinical Medicine, 10(16), 3683.

Zuo, Y., Wang, C., Sun, X., Hu, C., Liu, J., Hong, X., ... & Liu, Y. (2021). Identification of matrix metalloproteinase-10 as a key mediator of podocyte injury and proteinuria. Kidney International, 100(4), 837-849.

Matilla, L., Roncal, C., Ibarrola, J., Arrieta, V., García-Peña, A., Fernández-Celis, A., ... & López-Andrés, N. (2020). A role for MMP-10 (matrix metalloproteinase-10) in calcific aortic valve stenosis. Arteriosclerosis, thrombosis, and vascular biology, 40(5), 1370-1382.

Razai, A. S., Eckelman, B. P., & Salvesen, G. S. (2020). Selective inhibition of matrix metalloproteinase 10 (MMP10) with a single-domain antibody. Journal of Biological Chemistry, 295(8), 2464-2472.

Lv, Y. P., Cheng, P., Zhang, J. Y., Mao, F. Y., Teng, Y. S., Liu, Y. G., ... & Zhuang, Y. (2019). Helicobacter pylori–induced matrix metallopeptidase-10 promotes gastric bacterial colonization and gastritis. Science advances, 5(4), eaau6547.

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For research use only. Not intended for any clinical use.

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