Human MYL4 ELISA Kit (V2LY-0626-LY3811)

Name: Human MYL4 ELISA Kit
SKU: V2LY-0626-LY3811
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.024 ng/mL

Detection Range

0.05-20 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

21.6 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Myosin Light Chain 4

Function

Regulatory light chain of myosin. Does not bind calcium.

Biological Process

Cardiac muscle contraction Source: BHF-UCL
Positive regulation of ATP-dependent activity Source: BHF-UCL
Regulation of the force of heart contraction Source: BHF-UCL

Cellular Location

Cytoskeleton
myosin II
Cytosol
Other locations
A band

Involvement in disease

Atrial fibrillation, familial, 18 (ATFB18):
A familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.

Zhong, Y., Tang, K., Nattel, S., Zhai, M., Gong, S., Yu, Q., ... & Li, H. (2023). Myosin light-chain 4 gene-transfer attenuates atrial fibrosis while correcting autophagic flux dysregulation. Redox Biology, 60, 102606.

Yu, Y., Fang, R., Jin, H., Wang, B., Gao, F., & He, B. (2023). Correlation between Serum Myosin Light Chain 4 Levels and Recurrence after Radiofrequency Ablation in Patients with Atrial Fibrillation. International Heart Journal, 64(4), 632-640.

Xu, X., Yu, Z., Ai, N., Liufu, S., Liu, X., Chen, B., ... & Yin, Y. (2023). Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs. Genes, 14(6), 1267.

Zhong, Y., Tang, K., Li, H., Zhao, D., Kou, W., Xu, S., ... & Xu, Y. (2019). Rs4968309 in myosin light chain 4 (MYL4) associated with atrial fibrillation onset and predicts clinical outcomes after catheter ablation in atrial fibrillation patients without structural heart disease. Circulation Journal, 83(10), 1994-2001.

Wang, T. Y., Arking, D. E., Maleszewski, J. J., Fox-Talbot, K., Nieuwenhuis, T. O., Santhanam, L., ... & Halushka, M. K. (2019). Human cardiac myosin light chain 4 (MYL4) mosaic expression patterns vary by sex. Scientific reports, 9(1), 12681.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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