Human NLRP2 ELISA Kit (V2LY-0626-LY5924)

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Basic Information

Sensitivity
0.0051 ng/mL
Detection Range
0.01-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
120.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
NLR family, pyrin domain containing 2
Function
Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature pro-inflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of pro-inflammatory caspases.
Biological Process
Apoptotic processIEA:UniProtKB-KW
Inflammatory responseIEA:UniProtKB-KW
Innate immune responseIEA:UniProtKB-KW
Negative regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIGI:UniProtKB
Positive regulation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIDA:HGNC-UCL
Positive regulation of interleukin-1 beta productionManual Assertion Based On ExperimentIDA:HGNC-UCL
Cellular Location
Cytoplasm

Zhang, T., Xing, F., Qu, M., Yang, Z., Liu, Y., Yao, Y., & Xing, N. (2023). NLRP2 in health and disease. Immunology.

Zhang, M., Wu, Y., Gao, R., Chen, X., Chen, R., & Chen, Z. (2022). Glucagon-like peptide-1 analogs mitigate neuroinflammation in Alzheimer's disease by suppressing NLRP2 activation in astrocytes. Molecular and Cellular Endocrinology, 542, 111529.

Li, T., Li, X., Mao, R., Pan, L., Que, Y., Zhu, C., ... & Li, S. (2022). NLRP2 inhibits cell proliferation and migration by regulating EMT in lung adenocarcinoma cells. Cell Biology International, 46(4), 588-598.

Ducza, L., Szücs, P., Hegedűs, K., Bakk, E., Gajtkó, A., Wéber, I., & Holló, K. (2021). NLRP2 is overexpressed in spinal astrocytes at the peak of mechanical pain sensitivity during complete freund adjuvant-induced persistent pain. International journal of molecular sciences, 22(21), 11408.

Zhang, Q., Sun, Y., He, Z., Xu, Y., Li, X., Ding, J., ... & Hu, G. (2020). Kynurenine regulates NLRP2 inflammasome in astrocytes and its implications in depression. Brain, Behavior, and Immunity, 88, 471-481.

Rossi, M. N., Pascarella, A., Licursi, V., Caiello, I., Taranta, A., Rega, L. R., ... & Prencipe, G. (2019). NLRP2 regulates proinflammatory and antiapoptotic responses in proximal tubular epithelial cells. Frontiers in Cell and Developmental Biology, 7, 252.

Mu, J., Wang, W., Chen, B., Wu, L., Li, B., Mao, X., ... & Wang, L. (2019). Mutations in NLRP2 and NLRP5 cause female infertility characterised by early embryonic arrest. Journal of Medical Genetics, 56(7), 471-480.

Matsuoka, Y., Yamashita, A., Matsuda, M., Kawai, K., Sawa, T., & Amaya, F. (2019). NLRP2 inflammasome in dorsal root ganglion as a novel molecular platform that produces inflammatory pain hypersensitivity. Pain, 160(9), 2149-2160.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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