Human NPNT ELISA Kit (V2LY-0626-LY4936)

Name: Human NPNT ELISA Kit
SKU: V2LY-0626-LY4936
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.029 ng/mL

Detection Range

0.05-20 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

61.9 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Nephronectin

Function

Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins (By similarity).

Biological Process

Branching involved in ureteric bud morphogenesisBy SimilarityISS:BHF-UCL
Cell-cell adhesion mediated by integrinBy SimilarityISS:BHF-UCL
Cell-matrix adhesionBy SimilarityISS:BHF-UCL
Cellular response to tumor necrosis factorBy SimilarityISS:BHF-UCL
Establishment of protein localizationIEA:Ensembl
Extracellular matrix organizationBy SimilarityISS:BHF-UCL
Pilomotor reflexIEA:Ensembl
Positive regulation of alkaline phosphatase activityIEA:Ensembl
Positive regulation of cell-substrate adhesionBy SimilarityISS:BHF-UCL
Positive regulation of ERK1 and ERK2 cascadeBy SimilarityISS:BHF-UCL
Positive regulation of osteoblast differentiationBy SimilarityISS:BHF-UCL
Positive regulation of smooth muscle contractionIEA:Ensembl
Positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiationIEA:Ensembl
Positive regulation of transforming growth factor beta receptor signaling pathwayIEA:Ensembl
Ureteric bud developmentBy SimilarityISS:BHF-UCL

Cellular Location

Secreted, extracellular space, extracellular matrix
Trapped on the cell surface or in the extracellular matrix.

Garis, M., Meyer, M. D., & Lwigale, P. (2024). Expression of Nephronectin in the Descemet's membrane of mouse corneas during development and adult homeostasis. Experimental Eye Research, 240, 109797.

Honda, M., Segawa, T., Ishikawa, K., Maeda, M., Saito, Y., & Kon, S. (2022). Nephronectin influences EAE development by regulating the Th17/Treg balance via reactive oxygen species. American Journal of Physiology-Cell Physiology, 322(4), C699-C711.

Sopel, N., Ohs, A., Schiffer, M., & Müller-Deile, J. (2022). A tight control of non-canonical TGF-β pathways and MicroRNAs downregulates nephronectin in podocytes. Cells, 11(1), 149.

Al‐Hamed, M. H., Altuwaijri, N., Alsahan, N., Ali, W., Abdulwahab, F., Alzahrani, F., ... & Alkuraya, F. S. (2022). A null founder variant in NPNT, encoding nephronectin, causes autosomal recessive renal agenesis. Clinical Genetics, 102(1), 61-65.

Müller-Deile, J., Sopel, N., Ohs, A., Rose, V., Gröner, M., Wrede, C., ... & Schiffer, M. (2021). Glomerular endothelial cell-derived microRNA-192 regulates nephronectin expression in idiopathic membranous glomerulonephritis. Journal of the American Society of Nephrology, 32(11), 2777-2794.

Magnussen, S. N., Toraskar, J., Hadler-Olsen, E., Steigedal, T. S., & Svineng, G. (2021). Nephronectin as a matrix effector in cancer. Cancers, 13(5), 959.

Sarfstein, R., Lapkina-Gendler, L., Nagaraj, K., Laron, Z., & Werner, H. (2020). Identification of nephronectin as a new target for IGF1 action. European Journal of Cancer, 141, 115-127.

Magnussen, S. N., Toraskar, J., Wilhelm, I., Hasko, J., Figenschau, S. L., Molnar, J., ... & Svineng, G. (2020). Nephronectin promotes breast cancer brain metastatic colonization via its integrin-binding domains. Scientific reports, 10(1), 12237.

Lee, S., Honda, M., Yamamoto, S., Kumagai-Takei, N., Yoshitome, K., Nishimura, Y., ... & Otsuki, T. (2019). Role of nephronectin in pathophysiology of silicosis. International journal of molecular sciences, 20(10), 2581.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-NPNT Recombinant Antibody (CBWJN-1503) (CAT#: CBMAB-N3164-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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