Human NRDC ELISA Kit (V2LY-0626-LY5566)

Name: Human NRDC ELISA Kit
SKU: V2LY-0626-LY5566
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.016 ng/mL

Detection Range

0.05-10 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

131.7 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Nardilysin Convertase

Function

Cleaves peptide substrates on the N-terminus of arginine residues in dibasic pairs.

Biological Process

Negative regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Positive regulation of membrane protein ectodomain proteolysisManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of endopeptidase activityManual Assertion Based On ExperimentIDA:BHF-UCL

Cellular Location

cell surface
cytosol
mitochondrion

Oshima, S., Sinha, R., Ohno, M., Nishi, K., Eto, K., Takaori-Kondo, A., ... & Yamamoto, R. (2024). Nardilysin determines hematopoietic stem cell fitness by regulating protein synthesis. Biochemical and Biophysical Research Communications, 693, 149355.

Ohno, M., Shiomi, H., Baba, O., Yano, M., Aizawa, T., Nakano-Matsumura, Y., ... & Nishi, E. (2024). Auxiliary roles of nardilysin in the early diagnosis of acute coronary syndrome: a prospective cohort study, the Nardi-ACS study. Internal and Emergency Medicine, 1-11.

Saijo, S., Ohno, M., Iwasaki, H., Matsuda, S., Nishi, K., Hiraoka, Y., ... & Nishi, E. (2022). Nardilysin in adipocytes regulates UCP1 expression and body temperature homeostasis. Scientific Reports, 12(1), 3449.

Xu, Y., Xiong, Q., Yang, Y., Weng, N., Li, J., Liu, J., ... & Zhu, Q. (2022). Serum nardilysin as a prognostic biomarker in pancreatic ductal adenocarcinoma. Journal of Clinical Medicine, 11(11), 3101.

Fu, J., Ling, J., Li, C. F., Tsai, C. L., Yin, W., Hou, J., ... & Chiao, P. J. (2022). Nardilysin-Regulated Scission Mechanism Activates Polo-like Kinase 3 to Suppress the Development of Pancreatic Cancer. bioRxiv, 2022-07.

Yasuda, D., Hiraoka, Y., Ohno, M., Nishi, K., Iwasaki, H., Kita, T., ... & Kume, N. (2021). Deficiency of Nardilysin in the Liver Reduces Serum Cholesterol Levels. Biological and Pharmaceutical Bulletin, 44(3), 363-371.

Yoh, T., Hatano, E., Kasai, Y., Fuji, H., Nishi, K., Toriguchi, K., ... & Nishi, E. (2019). Serum nardilysin, a surrogate marker for epithelial–mesenchymal transition, predicts prognosis of intrahepatic cholangiocarcinoma after surgical resection. Clinical Cancer Research, 25(2), 619-628.

Ikuta, K., Fukuda, A., Ogawa, S., Masuo, K., Goto, N., Hiramatsu, Y., ... & Seno, H. (2019). Nardilysin inhibits pancreatitis and suppresses pancreatic ductal adenocarcinoma initiation in mice. Gut, 68(5), 882-892.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-NRDC Recombinant Antibody (A-8) (CAT#: CBMAB-N3561-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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