Human PADI2 ELISA Kit (2) (V2LY-0626-LY5656)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0035 ng/mL
Detection Range
0.007-1.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
75.6 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
peptidyl arginine deiminase, type II
Function
Catalyzes the deimination of arginine residues of proteins.
Biological Process
Cellular response to leukemia inhibitory factorIEA:Ensembl
Epigenetic maintenance of chromatin in transcription-competent conformationManual Assertion Based On ExperimentIMP:UniProtKB
Histone citrullinationManual Assertion Based On ExperimentIBA:GO_Central
Histone H3-R26 citrullinationManual Assertion Based On ExperimentIDA:UniProtKB
Intracellular estrogen receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of chemokine-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of lymphocyte chemotaxisManual Assertion Based On ExperimentIDA:UniProtKB
Protein citrullinationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of chromatin disassemblyManual Assertion Based On ExperimentIDA:UniProtKB
Substantia nigra developmentManual Assertion Based On ExperimentHEP:UniProtKB
Cellular Location
Cytoplasm

Nava-Quiroz, K. J., Rojas-Serrano, J., Pérez-Rubio, G., Buendia-Roldan, I., Mejía, M., Fernández-López, J. C., ... & Falfán-Valencia, R. (2023). Molecular factors in PAD2 (PADI2) and PAD4 (PADI4) are associated with interstitial lung disease susceptibility in rheumatoid arthritis patients. Cells, 12(18), 2235.

Ballasy, N. N., Bering, E. A., Kokorudz, C., Radford, B. N., Zhao, X., Dean, W., & Hemberger, M. (2022). Padi2/3 deficiency alters the epigenomic landscape and causes premature differentiation of mouse trophoblast stem cells. Cells, 11(16), 2466.

Kim, H. J., Kim, W. J., Shin, H. R., Yoon, H. I., Moon, J. I., Lee, E., ... & Ryoo, H. M. (2022). ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation. Cellular and Molecular Life Sciences, 79(3), 155.

Ma, X., Wang, G., Fan, H., Li, Z., Chen, W., Xiao, J., ... & Yang, L. (2022). Long noncoding RNA FAM225A promotes the malignant progression of gastric cancer through the miR-326/PADI2 axis. Cell Death Discovery, 8(1), 20.

Sams, K. L., Mukai, C., Marks, B. A., Mittal, C., Demeter, E. A., Nelissen, S., ... & Coonrod, S. A. (2022). Delayed puberty, gonadotropin abnormalities and subfertility in male Padi2/Padi4 double knockout mice. Reproductive Biology and Endocrinology, 20(1), 150.

Xue, T., Liu, X., Zhang, M., E, Q., Liu, S., Zou, M., ... & Zhang, X. (2021). PADI2‐catalyzed MEK1 citrullination activates ERK1/2 and promotes IGF2BP1‐mediated SOX2 mRNA stability in endometrial cancer. Advanced Science, 8(6), 2002831.

Guzmán-Guzmán, I. P., Ramírez-Vélez, C. I., Falfán-Valencia, R., Navarro-Zarza, J. E., Gutiérrez-Pérez, I. A., Zaragoza-García, O., & Parra-Rojas, I. (2021). PADI2 polymorphisms are significantly associated with rheumatoid arthritis, autoantibodies serologic status and joint damage in women from southern Mexico. Frontiers in Immunology, 12, 718246.

Beato, M., & Sharma, P. (2020). Peptidyl arginine deiminase 2 (PADI2)-mediated arginine citrullination modulates transcription in cancer. International Journal of Molecular Sciences, 21(4), 1351.

Liu, L., Zhang, Z., Zhang, G., Wang, T., Ma, Y., & Guo, W. (2020). Down-regulation of PADI2 prevents proliferation and epithelial-mesenchymal transition in ovarian cancer through inhibiting JAK2/STAT3 pathway in vitro and in vivo, alone or in combination with Olaparib. Journal of translational medicine, 18, 1-19.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PADI2, Active protein, GST Tag (CAT#: V2LY-0526-LY5942)

Mouse Recombinant PADI2, Active protein, GST Tag (CAT#: V2LY-0526-LY8737)

Mouse Padi2 ELISA Kit (CAT#: V2LY-0626-LY2961)

Human PADI2 ELISA Kit (CAT#: V2LY-0626-LY5128)

Related Products

Human PADI2 ELISA Kit (CAT#: V2LY-0626-LY5128)

Mouse Recombinant PADI2, Active protein, GST Tag (CAT#: V2LY-0526-LY8737)

Human Anti-Padi2 Recombinant Antibody (2121) (CAT#: CBMAB-P0638-YC)

Mouse Padi2 ELISA Kit (CAT#: V2LY-0626-LY2961)

Mouse Anti-PADI2 Recombinant Antibody (2D8C12) (CAT#: CBMAB-P0639-YC)

Mouse Anti-PADI2 Recombinant Antibody (CBLNP-202) (CAT#: CBMAB-1643-CN)

Mouse Anti-PADI2 Recombinant Antibody (4D4) (CAT#: CBMAB-P0640-YC)

Human Recombinant PADI2, Active protein, GST Tag (CAT#: V2LY-0526-LY5942)

Isotype control

For research use only. Not intended for any clinical use.

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