Human PAK3 ELISA Kit (V2LY-0626-LY5224)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0073 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
62.3 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
p21 protein (Cdc42/Rac)-activated kinase 3
Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (By similarity).
Biological Process
AxonogenesisISS:UniProtKB
Cellular response to organic cyclic compoundIEA:Ensembl
Dendrite developmentISS:UniProtKB
Dendritic spine morphogenesisISS:UniProtKB
Ephrin receptor signaling pathwayTAS:Reactome
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of dendritic spine morphogenesisIEA:Ensembl
Positive regulation of DNA biosynthetic processIEA:Ensembl
Positive regulation of fibroblast migrationIEA:Ensembl
Positive regulation of neuron apoptotic processIEA:Ensembl
Protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of actin filament polymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of axonogenesisManual Assertion Based On ExperimentIBA:GO_Central
Regulation of MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Stimulatory C-type lectin receptor signaling pathwayTAS:Reactome
Synapse organizationManual Assertion Based On ExperimentTAS:UniProtKB
Cellular Location
Cytoplasm
Involvement in disease
Intellectual developmental disorder, X-linked 30 (XLID30):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation, while syndromic mental retardation presents with associated physical, neurological and/or psychiatric manifestations.
PTM
Autophosphorylated when activated by CDC42/p21.
Neddylated.

Dobrigna, M., Poëa-Guyon, S., Rousseau, V., Vincent, A., Toutain, A., & Barnier, J. V. (2023). The molecular basis of p21-activated kinase-associated neurodevelopmental disorders: From genotype to phenotype. Frontiers in Neuroscience, 17, 1123784.

Liu, H., Liu, K., & Dong, Z. (2021). The role of p21-activated kinases in cancer and beyond: where are we heading?. Frontiers in cell and developmental biology, 9, 641381.

Bautista, L., Knippler, C. M., & Ringel, M. D. (2020). p21-Activated kinases in thyroid cancer. Endocrinology, 161(8), bqaa105.

Wu, H. Y., Yang, M. C., Ding, L. Y., Chen, C. S., & Chu, P. C. (2019). p21-Activated kinase 3 promotes cancer stem cell phenotypes through activating the Akt-GSK3β-β-catenin signaling pathway in pancreatic cancer cells. Cancer letters, 456, 13-22.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PAK3, Active protein, GST Tag (CAT#: V2LY-0526-LY5954)

Human Recombinant PAK3 protein, His Tag (CAT#: V2LY-0526-LY5955)

Mouse Recombinant PAK3, Active protein, GST Tag (CAT#: V2LY-0526-LY8738)

Related Products

Human Recombinant PAK3, Active protein, GST Tag (CAT#: V2LY-0526-LY5954)

Rabbit Anti-PAK3 Recombinant Antibody (EP797Y) (CAT#: CBMAB-1648-CN)

Mouse Anti-PAK3 Recombinant Antibody (CBT2500) (CAT#: V2LY-0625-LY633)

Rabbit Anti-PAK3 Recombinant Antibody (7i45) (CAT#: CBMAB-P0701-YC)

Human Recombinant PAK3 protein, His Tag (CAT#: V2LY-0526-LY5955)

Mouse Anti-PAK3 Recombinant Antibody (3A12) (CAT#: CBMAB-A6308-LY)

Rabbit Anti-PAK3 Recombinant Antibody (CBYC-P147) (CAT#: CBMAB-P0703-YC)

Mouse Anti-PAK3 Recombinant Antibody (CBT2833) (CAT#: V2LY-0625-LY632)

Mouse Recombinant PAK3, Active protein, GST Tag (CAT#: V2LY-0526-LY8738)

Mouse Anti-PAK3 Recombinant Antibody (1H7) (CAT#: CBMAB-P0700-YC)

Isotype control

For research use only. Not intended for any clinical use.

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