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Mouse Anti-PAK3 Recombinant Antibody (1H7) (CBMAB-P0700-YC)

Provided herein is a Mouse monoclonal antibody against Human P21 (RAC1) Activated Kinase 3. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all PAK3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1H7
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
PAK3 (NP_002569, 1-90 aa) partial recombinant protein with GST tag. Immunogen sequence: MSDGLDNEEK PPAPPLRMNS NNRDSSALNH SSKPLPMAPE EKNKKARLRS IFPGGGDKTN KKKEKERPEI SLPSDFEHTI HVGFDAVTGE
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-90

Target

Full Name
p21 protein (Cdc42/Rac)-activated kinase 3
Introduction
PAK3 is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability.
Entrez Gene ID
5063
UniProt ID
O75914
Alternative Names
P21 (RAC1) Activated Kinase 3; P21 Protein (Cdc42/Rac)-Activated Kinase 3; P21 (CDKN1A)-Activated Kinase 3; Oligophrenin-3; EC 2.7.11.1; Beta-PAK; OPHN3; PAK-3; Serine/Threonine-Protein Kinase PAK 3; Adriamycin Resistance-Associated;
Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (By similarity).
Biological Process
AxonogenesisISS:UniProtKB
Cellular response to organic cyclic compoundIEA:Ensembl
Dendrite developmentISS:UniProtKB
Dendritic spine morphogenesisISS:UniProtKB
Ephrin receptor signaling pathwayTAS:Reactome
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of dendritic spine morphogenesisIEA:Ensembl
Positive regulation of DNA biosynthetic processIEA:Ensembl
Positive regulation of fibroblast migrationIEA:Ensembl
Positive regulation of neuron apoptotic processIEA:Ensembl
Protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of actin filament polymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of axonogenesisManual Assertion Based On ExperimentIBA:GO_Central
Regulation of MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Stimulatory C-type lectin receptor signaling pathwayTAS:Reactome
Synapse organizationManual Assertion Based On ExperimentTAS:UniProtKB
Cellular Location
Cytoplasm
Involvement in disease
Intellectual developmental disorder, X-linked 30 (XLID30):
A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation, while syndromic mental retardation presents with associated physical, neurological and/or psychiatric manifestations.
PTM
Autophosphorylated when activated by CDC42/p21.
Neddylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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