Human PCNP ELISA Kit (V2LY-0626-LY5014)

Name: Human PCNP ELISA Kit
SKU: V2LY-0626-LY5014
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0073 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

18.9 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

PEST Proteolytic Signal Containing Nuclear Protein

Function

May be involved in cell cycle regulation.

Biological Process

Cell cycleIEA:UniProtKB-KW
Proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:HGNC-UCL
Protein ubiquitinationManual Assertion Based On ExperimentIDA:HGNC-UCL

Cellular Location

Nucleus

PTM

Ubiquitinated; mediated by UHRF2 and leading to its subsequent proteasomal degradation.
N-terminally acetylated in a HYPK-dependent manner by the NatA acetyltransferase complex which is composed of NAA10 and NAA15.

Yan, D., Liu, Y., Zhang, S., Fang, B., Zhao, F., & Yang, Z. (2024). PCNP: A RoCEv2 congestion control using precise CNP. Computer Networks, 247, 110453.

Zhang, L., Guo, D., Shen, J., Zheng, Y., Zhai, J., Li, R., ... & Zhang, B. (2023). Tissue mechanics modulate PCNP expression in oral squamous cell carcinomas with different differentiation. Frontiers in Oncology, 12, 1072276.

Chen, Y. G., Liu, H. X., Hong, Y., Dong, P. Z., Liu, S. Y., Gao, Y. R., ... & Ji, X. Y. (2022). PCNP is a novel regulator of proliferation, migration, and invasion in human thyroid cancer. International Journal of Biological Sciences, 18(9), 3605.

Xu, T., Wu, K., Shi, J., Ji, L., Song, X., Tao, G., ... & Jiang, B. (2022). LINC00858 promotes colon cancer progression through activation of STAT3/5 signaling by recruiting transcription factor RAD21 to upregulate PCNP. Cell death discovery, 8(1), 228.

Caruso, A. M., & Schlamb, C. (2021). PCNP: A new era in shaping health policy. Journal of the American Association of Nurse Practitioners, 33(11), 857-861.

Han, Z. P., Yang, Y., Chen, H. Y., Zhong, M. Y., & Zhuang, G. H. (2021). TIPE2 and PCNP expression abnormalities in peripheral blood mononuclear cells associated with disease activity in rheumatoid arthritis: a meta-analysis. European Review for Medical & Pharmacological Sciences, 25(3).

Dong, P., Fu, H., Chen, L., Zhang, S., Zhang, X., Li, H., ... & Ji, X. (2020). PCNP promotes ovarian cancer progression by accelerating β‐catenin nuclear accumulation and triggering EMT transition. Journal of Cellular and Molecular Medicine, 24(14), 8221-8235.

Afzal, A., Sarfraz, M., Li, G. L., Ji, S. P., Duan, S. F., Khan, N. H., ... & Ji, X. Y. (2019). Taking a holistic view of PEST‐containing nuclear protein (PCNP) in cancer biology. Cancer Medicine, 8(14), 6335-6343.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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