Human PLB1 ELISA Kit (V2LY-0626-LY5028)

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Tested Data
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Basic Information

Sensitivity
0.0092 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
163.1 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Phospholipase B1
Function
Calcium-independent membrane-associated phospholipase that catalyzes complete diacylation of phospholipids by hydrolyzing both sn-1 and sn-2 fatty acyl chains attached to the glycerol backbone (phospholipase B activity) (By similarity).
Has dual phospholipase and lysophospholipase activities toward diacylphospholipids. Preferentially cleaves sn-2 ester bonds over sn-1 bonds. Acts as a lipase toward glycerolipid substrates (By similarity).
Hydrolyzes fatty acyl chains of diacylglycerols with preference for the sn-2 position and of triacylglycerols with not positional selectivity (By similarity).
May also hydrolyze long chain retinyl esters such as retinyl palmitate (By similarity).
May contribute to digestion of dietary phospholipids, glycerolipids and retinoids, facilitating lipid absorption at the brush border (By similarity).
Biological Process
Diacylglycerol catabolic processIEA:Ensembl
Phosphatidylcholine acyl-chain remodelingTAS:Reactome
Phosphatidylcholine catabolic processIEA:Ensembl
Phosphatidylethanolamine catabolic processIEA:Ensembl
Phosphatidylglycerol catabolic processIEA:Ensembl
Phospholipid metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of acrosome reactionIEA:Ensembl
Retinoid metabolic processTAS:Reactome
Triglyceride catabolic processIEA:Ensembl
Cellular Location
Apical cell membrane
Present in the intestinal brush border membranes.
Topology
Extracellular: 22-1417
Helical: 1418-1438
Cytoplasmic: 1439-1458
PTM
Undergoes proteolytic cleavage in the ileum.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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