Human RAP1B ELISA Kit (V2LY-0626-LY5165)

Name: Human RAP1B ELISA Kit
SKU: V2LY-0626-LY5165
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0095 ng/mL

Detection Range

0.02-3.8 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

20.8 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

RAP1B, Member Of RAS Oncogene Family

Function

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.

Biological Process

Biological Process calcium-ion regulated exocytosisIEA:Ensembl
Biological Process cell population proliferationIEA:Ensembl
Biological Process cellular response to cAMPManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process establishment of endothelial barrierManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process establishment of localization in cellIEA:Ensembl
Biological Process negative regulation of calcium ion-dependent exocytosisIEA:Ensembl
Biological Process negative regulation of synaptic vesicle exocytosisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of ERK1 and ERK2 cascadeIEA:Ensembl
Biological Process positive regulation of integrin activationManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process Rap protein signal transductionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of cell junction assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of establishment of cell polarityManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cell membrane
Cytoplasm, cytosol
Cell junction
May shuttle between plasma membrane and cytosol (PubMed:3141412).
Presence of KRIT1 and CDH5 is required for its localization to the cell junction (PubMed:20332120).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-RAP1B Recombinant Antibody (360) (CAT#: CBMAB-R1313-CN)

Rabbit Anti-RAP1B Recombinant Antibody (36E1) (CAT#: CBMAB-CP2209-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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