Human Recombinant AIM2 protein, GST Tag (V2LY-0526-LY1965)

Name: Human Recombinant AIM2 protein, GST Tag
SKU: V2LY-0526-LY1965
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant AIM2 protein, GST Tag consist of Amino Acid: 1-343 and predicts a molecular mass of 65.2 kDa.

Molecule Mass

65.2 kDa

Sequence

Amino Acid: 1-343

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Absent In Melanoma 2

Function

Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.

Biological Process

Activation of innate immune response Source: UniProtKB
Apoptotic process Source: UniProtKB-KW
Cellular response to drug Source: MGI
Cellular response to interferon-beta Source: GO_Central
Immune response Source: ProtInc
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
NLRP3 inflammasome complex assembly Source: UniProtKB
Positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
Positive regulation of defense response to virus by host Source: UniProtKB
Positive regulation of interleukin-1 beta production Source: UniProtKB
Positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
Pyroptosis Source: UniProtKB
Pyroptosome complex assembly Source: GO_Central
Tumor necrosis factor-mediated signaling pathway Source: UniProtKB

Cellular Location

Cytoplasm; Nucleus. Activated inflammasomes can aggregate in the cytosol as speck-like particles.

Figueira, M. B. D. A., de Lima, D. S., Boechat, A. L., Antunes, I. A., Matsuda, J. D. S., Ribeiro, T. R. D. A., ... & Sadahiro, A. (2021). Single-Nucleotide Variants in the AIM2–Absent in Melanoma 2 Gene (rs1103577) Associated With Protection for Tuberculosis. Frontiers in immunology, 12, 824.

Li, Y. K., Chen, J. G., & Wang, F. (2021). The emerging roles of absent in melanoma 2 (AIM2) inflammasome in central nervous system disorders. Neurochemistry International, 105122.

Vakrakou, A. G., Svolaki, I. P., Evangelou, K., Gorgoulis, V. G., & Manoussakis, M. N. (2020). Cell-autonomous epithelial activation of AIM2 (absent in melanoma-2) inflammasome by cytoplasmic DNA accumulations in primary Sjögren's syndrome. Journal of autoimmunity, 108, 102381.

Yang, Y., Zhang, M., Jin, C., Ding, Y., Yang, M., Wang, R., ... & Hu, R. (2019). Absent in melanoma 2 suppresses epithelial‐mesenchymal transition via Akt and inflammasome pathways in human colorectal cancer cells. Journal of cellular biochemistry, 120(10), 17744-17756.

Zhu, W., Zu, X., Liu, S., & Zhang, H. (2019). The absent in melanoma 2 (AIM2) inflammasome in microbial infection. Clinica Chimica Acta, 495, 100-108.

Choubey, D. (2019). Type I interferon (IFN)-inducible Absent in Melanoma 2 proteins in neuroinflammation: implications for Alzheimer’s disease. Journal of neuroinflammation, 16(1), 1-10.

Shi, X., Wang, L., Ren, L., Li, J., Li, S., Cui, Q., & Li, S. (2019). Dihydroartemisinin, an antimalarial drug, induces absent in melanoma 2 inflammasome activation and autophagy in human hepatocellular carcinoma HepG2215 cells. Phytotherapy Research, 33(5), 1413-1425.

Zhang, Z., Dong, X., Yang, X., Wan, D., Sun, L., Gu, M., ... & He, S. (2017). Expression and clinical significance of absent in melanoma 2 in colorectal cancer. Biomedicine & Pharmacotherapy, 94, 843-849.

Choubey, D., & Panchanathan, R. (2017). Absent in Melanoma 2 proteins in SLE. Clinical immunology, 176, 42-48.

Sun, Q., Loughran, P., Shapiro, R., Shrivastava, I. H., Antoine, D. J., Li, T., ... & Scott, M. J. (2017). Retracted: Redox‐dependent regulation of hepatocyte absent in melanoma 2 inflammasome activation in sterile liver injury in mice. Hepatology, 65(1), 253-268.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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