Mouse Anti-AIM2 Recombinant Antibody (V2-180353) (CBMAB-A1813-YC)

Name: Mouse Anti-AIM2 Recombinant Antibody (V2-180353)
SKU: CBMAB-A1813-YC
Rating: 5 (1 reviews)

See all AIM2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180353

Application

WB, IF, ICC

Immunogen

Recombinant fragment of human AIM2 expressed in E. Coli

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:2,000
IF(ICC)	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Absent In Melanoma 2

Introduction

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.

Entrez Gene ID

9447

UniProt ID

O14862

Alternative Names

Absent In Melanoma 2; Interferon-Inducible Protein AIM2; PYHIN4;

Function

Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.

Biological Process

Activation of innate immune response Source: UniProtKB
Apoptotic process Source: UniProtKB-KW
Cellular response to drug Source: MGI
Cellular response to interferon-beta Source: GO_Central
Immune response Source: ProtInc
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
NLRP3 inflammasome complex assembly Source: UniProtKB
Positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
Positive regulation of defense response to virus by host Source: UniProtKB
Positive regulation of interleukin-1 beta production Source: UniProtKB
Positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
Pyroptosis Source: UniProtKB
Pyroptosome complex assembly Source: GO_Central
Tumor necrosis factor-mediated signaling pathway Source: UniProtKB

Cellular Location

Cytoplasm; Nucleus. Activated inflammasomes can aggregate in the cytosol as speck-like particles.

Figueira, M. B. D. A., de Lima, D. S., Boechat, A. L., Antunes, I. A., Matsuda, J. D. S., Ribeiro, T. R. D. A., ... & Sadahiro, A. (2021). Single-Nucleotide Variants in the AIM2–Absent in Melanoma 2 Gene (rs1103577) Associated With Protection for Tuberculosis. Frontiers in immunology, 12, 824.

Li, Y. K., Chen, J. G., & Wang, F. (2021). The emerging roles of absent in melanoma 2 (AIM2) inflammasome in central nervous system disorders. Neurochemistry International, 105122.

Vakrakou, A. G., Svolaki, I. P., Evangelou, K., Gorgoulis, V. G., & Manoussakis, M. N. (2020). Cell-autonomous epithelial activation of AIM2 (absent in melanoma-2) inflammasome by cytoplasmic DNA accumulations in primary Sjögren's syndrome. Journal of autoimmunity, 108, 102381.

Yang, Y., Zhang, M., Jin, C., Ding, Y., Yang, M., Wang, R., ... & Hu, R. (2019). Absent in melanoma 2 suppresses epithelial‐mesenchymal transition via Akt and inflammasome pathways in human colorectal cancer cells. Journal of cellular biochemistry, 120(10), 17744-17756.

Zhu, W., Zu, X., Liu, S., & Zhang, H. (2019). The absent in melanoma 2 (AIM2) inflammasome in microbial infection. Clinica Chimica Acta, 495, 100-108.

Choubey, D. (2019). Type I interferon (IFN)-inducible Absent in Melanoma 2 proteins in neuroinflammation: implications for Alzheimer’s disease. Journal of neuroinflammation, 16(1), 1-10.

Shi, X., Wang, L., Ren, L., Li, J., Li, S., Cui, Q., & Li, S. (2019). Dihydroartemisinin, an antimalarial drug, induces absent in melanoma 2 inflammasome activation and autophagy in human hepatocellular carcinoma HepG2215 cells. Phytotherapy Research, 33(5), 1413-1425.

Zhang, Z., Dong, X., Yang, X., Wan, D., Sun, L., Gu, M., ... & He, S. (2017). Expression and clinical significance of absent in melanoma 2 in colorectal cancer. Biomedicine & Pharmacotherapy, 94, 843-849.

Choubey, D., & Panchanathan, R. (2017). Absent in Melanoma 2 proteins in SLE. Clinical immunology, 176, 42-48.

Sun, Q., Loughran, P., Shapiro, R., Shrivastava, I. H., Antoine, D. J., Li, T., ... & Scott, M. J. (2017). Retracted: Redox‐dependent regulation of hepatocyte absent in melanoma 2 inflammasome activation in sterile liver injury in mice. Hepatology, 65(1), 253-268.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-AIM2 Recombinant Antibody (V2-180353)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-AIM2 Recombinant Antibody (V2-180352) (CAT#: CBMAB-A1812-YC)

Rabbit Anti-AIM2 Recombinant Antibody (V2-180356) (CAT#: CBMAB-A1816-YC)

Mouse Anti-AIM2 Recombinant Antibody (V2-180354) (CAT#: CBMAB-A1814-YC)

Mouse Anti-AIM2 Recombinant Antibody (CBT3645) (CAT#: V2LY-0625-LY310)

Mouse Anti-AIM2 Recombinant Antibody (V2-180355) (CAT#: CBMAB-A1815-YC)

Mouse Anti-AIM2 Recombinant Antibody (V2-180349) (CAT#: CBMAB-A1809-YC)

Mouse Anti-AIM2 Recombinant Antibody (V2-180351) (CAT#: CBMAB-A1811-YC)

Mouse Anti-AIM2 Recombinant Antibody (CBT3179) (CAT#: V2LY-0625-LY369)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry